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CHEK1 Antikörper

CHEK1 Reaktivität: Human WB, IF, IHC (p), ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN2857050
  • Target Alle CHEK1 Antikörper anzeigen
    CHEK1 (Checkpoint Kinase 1 (CHEK1))
    Reaktivität
    • 215
    • 119
    • 68
    • 16
    • 9
    • 9
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Human
    Wirt
    • 224
    • 14
    • 7
    • 5
    Kaninchen
    Klonalität
    • 210
    • 41
    Polyklonal
    Konjugat
    • 135
    • 13
    • 11
    • 9
    • 9
    • 9
    • 9
    • 8
    • 8
    • 8
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    Dieser CHEK1 Antikörper ist unkonjugiert
    Applikation
    • 173
    • 87
    • 54
    • 41
    • 40
    • 36
    • 35
    • 17
    • 16
    • 13
    • 10
    • 7
    • 6
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    Kreuzreaktivität
    Human
    Produktmerkmale
    Rabbit Polyclonal antibody to Chk1 (CHK1 checkpoint homolog (S. pombe))
    Chk1 antibody [C1C2-6], Internal
    Aufreinigung
    Purified by antigen-affinity chromatography.
    Immunogen
    Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Chk1. The exact sequence is proprietary.
    Isotyp
    IgG
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  • Applikationshinweise
    WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal
    Konservierungsmittel
    Thimerosal (Merthiolate)
    Vorsichtsmaßnahmen
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target
    CHEK1 (Checkpoint Kinase 1 (CHEK1))
    Andere Bezeichnung
    checkpoint kinase 1 (CHEK1 Produkte)
    Synonyme
    An08g10320 antikoerper, AO090003000441 antikoerper, CHK1 antikoerper, chk1 antikoerper, C85740 antikoerper, Chk1 antikoerper, rad27 antikoerper, id:ibd2720 antikoerper, zgc:56093 antikoerper, CHEK1 antikoerper, checkpoint kinase 1 antikoerper, serine/threonine-protein kinase chk1 antikoerper, serine/threonine-protein kinase Chk1 antikoerper, CAMK/CAMKL/CHK1 protein kinase Chk1 antikoerper, checkpoint kinase 1 S homeolog antikoerper, CHEK1 antikoerper, ANI_1_2436074 antikoerper, AOR_1_768154 antikoerper, PTRG_04183 antikoerper, SJAG_01680 antikoerper, PAAG_04978 antikoerper, MCYG_03290 antikoerper, VDBG_03742 antikoerper, chek1.S antikoerper, Chek1 antikoerper, chek1 antikoerper
    Hintergrund
    Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

    Molekulargewicht
    54 kDa
    Gen-ID
    1111
    UniProt
    O14757
    Pathways
    p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur
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