NDUFA5 Antikörper (full length)

Produktnummer ABIN2856783

Produktdetails anti-NDUFA5 Antikörper

Target: NDUFA5 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 5, 13kDa (NDUFA5))

Bindungsspezifität: full length

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NDUFA5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Schimpanse

Kreuzreaktivität (Details): Chimpanzee (100 %)

Produktmerkmale: Rabbit Polyclonal antibody to NDUFA5 (NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13 kDa)
NDUFA5 antibody [N1C3]

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Full length human NDUFA5 Recombinant protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Kommentare:

Positive Control: H1299 , HeLa , HepG2 , mouse brain

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu NDUFA5

Target: NDUFA5 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 5, 13kDa (NDUFA5))

Andere Bezeichnung: NDUFA5 (NDUFA5 Produkte)

Synonyme: NADHUO antikoerper, B13 antikoerper, CI-13KD-B antikoerper, CI-13kB antikoerper, NUFM antikoerper, UQOR13 antikoerper, 2900002J19Rik antikoerper, NADH:ubiquinone oxidoreductase subunit A5 antikoerper, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 antikoerper, Ndufa5 antikoerper, NDUFA5 antikoerper

Hintergrund: The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes.

Cellular Localization: Mitochondrion inner membrane, Peripheral membrane protein, Matrix side

Molekulargewicht: 13 kDa

Gen-ID: 4698