PSMD14 Antikörper (Center)

Produktnummer ABIN2856031

Produktdetails anti-PSMD14 Antikörper

Target: PSMD14 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 14 (PSMD14))

Bindungsspezifität: Center

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PSMD14 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktmerkmale: Rabbit Polyclonal antibody to MAD1 (MAD1 mitotic arrest deficient-like 1 (yeast))
MAD1 antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MAD1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Kommentare:

Positive Control: 293T , A431 , HeLa , HepG2 , A375

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-PSMD14 Antikörper (ABIN2856031)

Alfonso-Pérez, Hayward, Holder, Gruneberg, Barr: "MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling." in: The Journal of cell biology, Vol. 218, Issue 4, pp. 1108-1117, (2020) (PubMed).

Details zu PSMD14

Target: PSMD14 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 14 (PSMD14))

Andere Bezeichnung: MAD1 (PSMD14 Produkte)

Synonyme: PAD1 antikoerper, POH1 antikoerper, RPN11 antikoerper, 2610312C03Rik antikoerper, 3200001M20Rik antikoerper, AA986732 antikoerper, Pad1 antikoerper, Poh1 antikoerper, rpm11 antikoerper, zgc:162272 antikoerper, proteasome 26S subunit, non-ATPase 14 antikoerper, proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 antikoerper, 26S proteasome non-ATPase regulatory subunit 14 antikoerper, proteasome 26S subunit, non-ATPase 14 L homeolog antikoerper, 26S proteasome-associated pad1 homolog antikoerper, PSMD14 antikoerper, Psmd14 antikoerper, rpn-11 antikoerper, psmd14 antikoerper, psmd14.L antikoerper

Hintergrund: MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene.

Cellular Localization: Nucleus , Kinetochore

Molekulargewicht: 83 kDa

Gen-ID: 8379

Pathways: Mitotic G1-G1/S Phases, DNA Replication, M Phase, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Ubiquitin Proteasome Pathway