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Vimentin Antikörper

Name: Vimentin Antikörper
Brand: antibodies-online
SKU: ABIN2854814
Price: 630.63 EUR
Availability: InStock

KO Validated VIM Reaktivität: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

WB Image Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody , diluted at 1:10000.

WB Image Vimentin antibody detects Vimentin protein by western blot analysis.

IHC-P Image Vimentin antibody detects Vimentin protein at cytoplasm in rat testis by immunohistochemical analysis.

IHC

IP Image Immunoprecipitation of Vimentin protein from HeLa whole cell extracts using 5 μg of Vimentin antibody, Western blot analysis was performed using Vimentin antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image Vimentin antibody detects Vimentin protein at cytosol on mouse thymus gland by immunohistochemical analysis.

IHC

IHC-Fr Image Immunofluorescence photomicrographs of frozen sections of mouse brain.

IHC

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Vimentin, antibody (Green) at 1:500 dilution.

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Vimentin, antibody at 1:500 dilution.

IHC

Effects of MCME on migration-related proteins in CL1-0 and CL1-5 cells.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Fusion progeny exhibit epithelial-mesenchymal transition(A) Representative phase-contrast microscopic images of fusion progeny and parental cells.

FOXF1 mediates reprogramming effects on fusion progeny(A) FOXF1 mRNA levels were determined by real-time PCR in fusion progeny and parent cell lines.

Expression pattern of Notch ligands and receptors in first trimester decidual tissue.

IF (cc)

MEF2B-V5 expression alters the abundance of protein from target genes and increases HEK293A cell migration.

MEF2B mutations alter the abundance of protein from MEF2B target genes and decrease cell migration.

Serum-free suspension culture induces the enrichment of Glioma stem-like cells (GSCs).

Aryl hydrocarbon receptor (AhR) expression in different cells was correlated with E-cadherin and vimentin expression and cell motility.

Level of autophagy-related protein expression in different cells was correlated with their cell motility.

Expression of epithelial-mesenchymal transition (EMT) markers in MCF-7 o/e Snail cells.

Differential expression of epithelial-mesenchymal transition (EMT) markers in MCF-7 o/e Snail cells and MCF-F o/e Snail tissues.

ZEB2-AS1 knockdown inhibits migration, invasion and EMT in HNSCC cells.

Reintroduction of exogenous ZEB2 largely attenuated the phenotypical changes induced by ZEB2-AS1 inhibition.

ZEB2-AS1 is involved for TGF-β1-induced EMT in HNSCC cells.

Lipid loading augments hypoxia mediated secretion of pro-tumorigenic factors.

ISX expression induces TWIST1 and Snail1 expression, and promotes EMTAThe expression levels of ISX, TWIST1, Snail1, fibronectin (FN1), VEGF, and E-cadherin (CDH1) mRNA were examined in a ISX-GFP inducible Tet-ON transformants at 8 h after addition of DOX.

ISX interacts with both BRD4 and PCAF in vitro and in vivoABRD4 peptide (291-314 a.

The expression of ISX, BRD4, and PCAF is correlated with lung cancer metastasisAIHC staining with ISX (brown) or PCAF (brown) antibody in lung tumors from patients with lung cancer.

Sequencing analysis of acetylated recombinant and trypsin-digested wild-type ISX using liquid chromatography-mass spectrometryA, BPCAF acetylation of ISX was detected at lysine residue 69 by liquid chromatography-tandem mass spectrometry (LC-tandem MS-MS).

Cataracts induced epithelial-mesenchymal transition (EMT) progression with significantly elevated N-cadherin and MMP9 expression in DM (+) patients.

IF (cc)

35 Abbildungen

(17 Referenzen)

Produktnummer ABIN2854814

Datenblatt (PDF)

Target Alle Vimentin (VIM) Antikörper anzeigen

Vimentin (VIM)
Reaktivität
332

127

126

61

59

44

43

37

30

22

20

14

13

8

7

6

6

4

3

2

1

1

1

1

1

1

1
Human
Wirt
188

130

9

6

4

4

2
Kaninchen
Passende Sekundärantikörper
Klonalität
196

146
Polyklonal
Konjugat
199

19

13

13

11

7

7

7

7

7

7

7

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
Dieser Vimentin Antikörper ist unkonjugiert
Applikation
281

132

98

97

95

64

48

40

39

27

23

19

8

5

4

4

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität

Cat, Hund, Human, Maus, Ratte

Produktmerkmale

Rabbit Polyclonal antibody to Vimentin (vimentin)
Vimentin antibody

Aufreinigung

Purified by antigen-affinity chromatography.

Güteklasse

KO Validated

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Isotyp

IgG

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anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
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anti-Vimentin (VIM) antibody
VIM Reaktivität: Human, Schwein, Rind (Kuh), Huhn, Hund, Cat, Ziege WB, IHC, FACS, StM Wirt: Maus Monoclonal VM452 unconjugated

anti-Vimentin (VIM) (AA 2-466) antibody
VIM Reaktivität: Human, Maus, Ratte WB, IHC, ELISA, FACS Wirt: Maus Monoclonal 3A1F2 unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human WB, IHC, StM Wirt: Kaninchen Monoclonal VIM-1937R unconjugated Recombinant Antibody

anti-Vimentin (VIM) (AA 41-140) antibody
VIM Reaktivität: Human WB, ELISA, IF, IP Wirt: Maus Monoclonal 3E9 unconjugated

Applikationshinweise

WB: 1:5000-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare

Positive Control: 293T
Validation: Comparison, KO/KD, Orthogonal, Overexpression

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.11 mg/mL

Buffer

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Navarrete-Bernal, Cervantes-Badillo, Martínez-Herrera, Lara-Torres, Gerson-Cwilich, Zentella-Dehesa, Ibarra-Sánchez, Esparza-López, Montesinos, Cortés-Morales, Osorio-Pérez, Villegas-Osorno et al.: "Biological Landscape of Triple Negative Breast Cancers Expressing CTLA-4. ..." in: Frontiers in oncology, Vol. 10, pp. 1206, (2020) (PubMed).

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Wang, Liu, Jeng, Chiang, Chai, Chiou, Huang, Yokoyama, Wang, Huang, Hsu: "PCAF-mediated acetylation of ISX recruits BRD4 to promote epithelial-mesenchymal transition." in: EMBO reports, Vol. 21, Issue 2, pp. e48795, (2020) (PubMed).

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Yin, Yin, Wang, Zhou, Liu, Yang, Wang, Lu: "MiR-206 suppresses epithelial mesenchymal transition by targeting TGF-β signaling in estrogen receptor positive breast cancer cells." in: Oncotarget, Vol. 7, Issue 17, pp. 24537-48, (2017) (PubMed).

Marquez-Vilendrer, Rai, Gramling, Lu, Reisman: "Loss of the SWI/SNF ATPase subunits BRM and BRG1 drives lung cancer development." in: Oncoscience, Vol. 3, Issue 11-12, pp. 322-336, (2016) (PubMed).

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Target

Vimentin (VIM)

Andere Bezeichnung

vimentin (VIM Produkte)

Synonyme

CTRCT30 antikoerper, cb28 antikoerper, vime antikoerper, vim antikoerper, vim1 antikoerper, vim2 antikoerper, VIM antikoerper, Vimentin antikoerper, vim4 antikoerper, vimentin antikoerper, vimentin L homeolog antikoerper, vimentin S homeolog antikoerper, VIM antikoerper, Vim antikoerper, vim antikoerper, vim.L antikoerper, vim.S antikoerper

Hintergrund

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Molekulargewicht

54 kDa

Gen-ID

7431

UniProt

P08670

Pathways

Caspase Kaskade in der Apoptose