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RAG1 Antikörper (AA 351-450) (Alexa Fluor 594)

RAG1 Reaktivität: Maus WB, FACS, IF (p), IF (cc) Wirt: Kaninchen Polyclonal Alexa Fluor 594
Produktnummer ABIN2806049
  • Target Alle RAG1 Antikörper anzeigen
    RAG1 (Recombination Activating Gene 1 (RAG1))
    Bindungsspezifität
    • 15
    • 2
    • 1
    • 1
    • 1
    AA 351-450
    Reaktivität
    • 18
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Wirt
    • 20
    • 1
    Kaninchen
    Klonalität
    • 20
    • 1
    Polyklonal
    Konjugat
    • 7
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser RAG1 Antikörper ist konjugiert mit Alexa Fluor 594
    Applikation
    • 21
    • 13
    • 12
    • 12
    • 4
    • 3
    • 3
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))
    Kreuzreaktivität
    Maus
    Homologie
    Human,Rat,Sheep,Pig
    Aufreinigung
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human RAG1/RNF74
    Isotyp
    IgG
    Top Product
    Discover our top product RAG1 Primärantikörper
  • Applikationshinweise
    FCM 1:20-100
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    RAG1 (Recombination Activating Gene 1 (RAG1))
    Andere Bezeichnung
    RNF74 (RAG1 Produkte)
    Synonyme
    RAG-1 antikoerper, rag1 antikoerper, RNF74 antikoerper, Rag-1 antikoerper, rag-1 antikoerper, rag1a antikoerper, recombination activating 1 antikoerper, recombination activating gene 1 antikoerper, recombination activating gene 1 S homeolog antikoerper, Rag1 antikoerper, RAG1 antikoerper, rag1 antikoerper, rag1.S antikoerper
    Hintergrund

    Synonyms: RAG-1, RNF74, V(D)J recombination-activating protein 1, RING finger protein 74, RAG1

    Background: Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination.

    Gen-ID
    5896
    UniProt
    P15918
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