FHL2 Antikörper

Produktnummer ABIN2763848

Produktdetails anti-FHL2 Antikörper

Target: FHL2 (Four and A Half LIM Domains 2 (FHL2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FHL2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Kreuzreaktivität: Human

Produktmerkmale: Four-and-a-half LIM only protein 2, FHL2,Sphingosine kinase-1 (SK1) is a key enzyme that catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P). SK1, and its product S1P, regulate numerous and diverse biological functions, including cell growth, differentiation, proliferation, and apoptosis. S1P also plays a central role in cardiac development and ischemic preconditioning. FHL2 (SLIM3) has been identified as a SK1-interacting protein in mammalian cardiomyocytes. FHL2, but not FHL1 or FHL3, interacts with SK1, and FHL2 colocalizes with SK1 in the cytoplasm of these cells. The interaction of FHL2 with SK-1 involves the C-terminal portion of SK1. Overexpression of FHL2 has been found to attneuate the activity and antiapoptotic effects of SK1. Endothelin-1, a potent survival factor in cardiomyocytes, inhibits FHL2-SK1 association and increases SK1 activity. FHL2 is therefore a novel inhibitor of SK1 activity in cardiomyocytes and targeting FHL2 may prevent myocardial apoptosis through activation of SK1. A transcriptional coactivator, FHL2 is also known to form complexes consisting of Proline-, glutamic acid-, and leucine-rich protein-1 (PELP1), FHL2, and androgen receptors (AR) in prostate cancer cells, perhaps functioning as a molecular adaptor with PELP1 in the progression of prostate cancer.

Aufreinigung: Antigen Immunoaffiinity Purification

Immunogen: Synthetic peptide derived from the human FHL2 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Antibody can be used for Western blotting (0.5-2 μg/mL). Also binds ABB73038, FHL2 isoform 5 with MW of 389 a.a. (~42 kDa). Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: Lot specific

Buffer: Provided as ligand affinity purified antibody in phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -80 °C

Informationen zur Lagerung: Product should be stored at -70°C. Aliquot to avoid freeze/thaw cycles

Details zu FHL2

Target: FHL2 (Four and A Half LIM Domains 2 (FHL2))

Andere Bezeichnung: FHL2 (FHL2 Produkte)

Synonyme: AAG11 antikoerper, DRAL antikoerper, FHL-2 antikoerper, SLIM-3 antikoerper, SLIM3 antikoerper, C76204 antikoerper, FHL2 antikoerper, zgc:92428 antikoerper, zgc:103466 antikoerper, four and a half LIM domains 2 antikoerper, four and a half LIM domains 2a antikoerper, four and a half LIM domains 2b antikoerper, FHL2 antikoerper, Fhl2 antikoerper, fhl2 antikoerper, fhl2a antikoerper, fhl2b antikoerper

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha