CD4 Antikörper (N-Term)

Produktnummer ABIN2747750

Produktdetails anti-CD4 Antikörper

Target: CD4

Bindungsspezifität: AA 270-320, N-Term

Reaktivität: Human, Maus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Spezifität: The antibody labels a strong band of CD4 at 55 kDa in ABIN1741637 samples & in other cancer cell lines.
The antibody also labels a band of 28 kDa, the identity ofthis band is not fully understood but may represent a proteolytic degradation product of CD4 in SKNMC cells.

Kreuzreaktivität: Maus, Ratte (Rattus), Affe

Kreuzreaktivität (Details): Does not cross react to any other cellular protein.

Produktmerkmale: CD4 selective antibodies were generated against a peptide taken from the human protein. The CD4 selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The CD4-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The supplier provides CD4 Western blot positive control samples in ready-to-use SDS-PAGE sample buffer. Limited quantities of antigenic blocking peptide for CD4 antibody is also available, please inquire before ordering.

Aufreinigung: Affinity Purified

Immunogen: Synthetic peptide taken within amino acid region 270-320 on human CD4 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.55-0.75 μg/μL

Handhabung: Working solutions of antibodies in buffer should be filtered through 0.45 μm filter after every use for long-term storage.

Lagerung: -20 °C

Informationen zur Lagerung: For long-term storage of keep at -20 °C. We don't recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer.

Details zu CD4

Target: CD4

Andere Bezeichnung: CD4 (CD4 Produkte)

Synonyme: cd4 antikoerper, L3T4 antikoerper, Ly-4 antikoerper, CD4mut antikoerper, W3/25 antikoerper, p55 antikoerper, CD4 molecule antikoerper, carcinoembryonic antigen-related cell adhesion molecule 5 antikoerper, T-cell surface glycoprotein CD4 antikoerper, CD4 antigen antikoerper, Cd4 molecule antikoerper, CD4 antikoerper, LOC100125525 antikoerper, cd4 antikoerper, Cd4 antikoerper

Hintergrund: Human T-cell surface glycoprotein CD4, also known as T-cell surface antigen T4/Leu-3, is a single chain trans membranous glycoprotein (55 kDa) which belongs to the immunoglobulin superfamily. CD4 (Cluster of Differentiation 4) consists of a cytoplasmic tail, four immunoglobulin-like external domains (D1-D4), and a transmembrane domain. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is present on a subset of T lymphocytes (helper/inducer T cells) and at a lower level on monocytes, regulatory T cells, tissue macrophages, granulocytes, Langerhans cells and dendritic cells. It is also expressed in specific regions of the brain. It is an important component of the immune system and is the cellular receptor for HIV-1. This protein initiates the early phase of T-cell activation, and is an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. CD4 plays a role in the regulation of T-B lymphocyte adhesion in the absence of antigen recognition, and in thymic differentiation. Subpopulations of suppressor or cytotoxic T cells are identified among CD4-positive T cells. The intracellular domain of the antigen is associated with p56 lck protein tyrosine kinase. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. The antigen interacts directly with major histocompatibility complex (MHC) class II molecules on the surface of the antigen presenting cell via its extracellular domain. It is also a major receptor for the human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors thus making the CD4 count an important factor to decide when to begin treatment for HIV-infected patients.The human CD4-specific gene maps to chromosome 12pter-p12.

NCBI Accession: NP_000607

UniProt: P01730

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events