Thromboxane A2 Receptor (TBXA2R) (1st Cytoplasmic Domain) Antikörper

Details zu Produkt Nr. ABIN270586, Anbieter: Anmelden zum Anzeigen
Antigen
  • tbxa2r
  • MGC80805
  • TBXA2R
  • BDPLT13
  • TXA2-R
  • TP
  • thromboxane A2 receptor L homeolog
  • thromboxane A2 receptor
  • tbxa2r.L
  • TBXA2R
  • tbxa2r
  • Tbxa2r
Epitop
1st Cytoplasmic Domain
6
4
3
3
2
2
2
1
1
1
1
1
Reaktivität
Human
41
7
6
3
2
1
Wirt
Kaninchen
39
3
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
23
15
14
9
4
3
2
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human TBXA2R.
Spezifität Human TBXA2R. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Reinigung Immunogen affinity purified
Antigen
Andere Bezeichnung TBXA2R (TBXA2R Antibody Abstract)
Hintergrund Gene Symbol: TBXA2R
Gen-ID 6915
UniProt P21731
Applikations-hinweise Immunohistochemistry-Paraffin 20 μg/mL
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for Thromboxane A2 receptor Antibody (NLS1277)Immunohistochemistry
1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
. Make 4 um sections and place on pre-cleaned and charged microscope slides.
. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each at RT.
. Rehydrate the tissues by washing the slides in 3 changes of 100 % alcohol for 3 minutes each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 3 minutes each at RT.
. Wash the slides in 1 change of 80 % alcohol for 3 minutes at RT.
. Rinse the slides in gentle running distilled water for 5 minutes at RT.
. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.
0) at 99-100 degrees Celciusfor 20 minutes.
. Remove the slides from the heat and let stand in buffer at RT for 20 minutes.
. Rinse the slides in 1X TBS-T for 1 minute at RT.**Do not allow the tissues to dry at any time during the staining procedure**
. Begin the immunostaining by applying a universal protein block for 20 minutes at RT.
. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase steptavidin for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase chromagen substrate for 30 minutes at RT.
. Rinse the slide in distilled water for 1 minute at RT.**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**
. Dehydrate the tissue by washing the slides in 2 changes of 80 % alcohol for 1 minute each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of 100 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of xylene for 1 minute each at RT.
. Apply cover slip.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.1 % Sodium Azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Bilder des Herstellers
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Thromboxane A2 Receptor (TBXA2R) (1st Cytoplasmic Domain) antibody (ABIN270586) Immunohistochemistry-Paraffin: Thromboxane A2 R/TBXA2R Antibody [NLS1277] - Analysis ...
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