CCL5 Antikörper (Chemokine (C-C Motif) Ligand 5)

Details for Product anti-CCL5 Antibody No. ABIN2689890, Anbieter: Anmelden zum Anzeigen
Antigen
  • Rantes
  • Scya5
  • D17S136E
  • RANTES
  • SCYA5
  • SIS-delta
  • SISd
  • TCP228
  • eoCP
  • MuRantes
  • C-C motif chemokine 5-like
  • C-C motif chemokine ligand 5
  • chemokine (C-C motif) ligand 5
  • LOC100230935
  • CCL5
  • Ccl5
Reaktivität
Human
202
112
55
26
14
4
4
4
3
2
1
1
1
1
1
Wirt
Maus
200
81
38
5
2
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CCL5 Antikörper ist unkonjugiert
55
24
14
10
8
6
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Blocking Antibody (Inhibition), Neutralization (Neut), Western Blotting (WB)
230
175
78
52
41
30
19
15
14
13
7
5
3
3
3
2
2
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Marke BD Pharmingen™
Immunogen Recombinant human RANTES protein
Klon 2D5
Isotyp IgG1, kappa
Produktmerkmale The 2D5 antibody reacts with human Regulated upon Activation, Normal T cells Expressed and Secreted (RANTES). The immunogen used to generated this hybridoma was recombinant human RANTES protein. This antibody is routinely tested by intracellular staining. Other applications were tested during antibody development only or reported in the literature. Expression of RANTES by cultured human PBMC. Human PBMC were cultured for 24 hours in the presence of BD GolgiStop™ (2 μM final concentration, Cat. No. 554724). The PBMC were harvested, stained with FITC-mouse anti-human CD3 antibody (FITC-HIT3a, Cat. No. 555339), fixed, permeabilized, and subsequently stained with 0.125 μg of PE-mouse anti-human RANTES antibody (PE-2D5, Cat. No. 554732) following BD Biosciences Pharmingen's staining protocol (left panel). To demonstrate specificity of staining, the binding of PE-2D5 antibody was blocked by the preincubation of the unlabeled 2D5 antibody (Cat. No. 556859) with fixed/permeabilized target cells prior to staining with the PE-2D5 antibody. The quadrant markers for the bivariate dot plots were set based on the auto-fluorescence control, and verified with unlabeled antibody blocking control (right panel).

BD Pharmingen™ Purified Mouse Anti-Human RANTES - Purified - Clone 2D5 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Plasmids, Primers & others Plasmide, Primers & weitere CCL5 products on genomics-online (e.g. as negative or positive controls)
Antigen Chemokine (C-C Motif) Ligand 5 (CCL5)
Andere Bezeichnung RANTES (CCL5 Antibody Abstract)
Pathways Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C.
Produkt verwendet in: Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).

Sticherling, Küpper, Koltrowitz, Bornscheuer, Kulke, Klinger, Wilhelm, Kameyoshi, Christophers, Schröder: "Detection of the chemokine RANTES in cytokine-stimulated human dermal fibroblasts." in: The Journal of investigative dermatology, Vol. 105, Issue 4, pp. 585-91, 1995 (PubMed).

Haben Sie etwas anderes gesucht?