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PIR-A/B (Extracellular Domain) Antikörper

Reaktivität: Maus FACS, IP Wirt: Ratte Monoclonal 6C1 unconjugated
Produktnummer ABIN2689880
  • Target
    PIR-A/B
    Bindungsspezifität
    Extracellular Domain
    Reaktivität
    Maus
    Wirt
    • 1
    Ratte
    Klonalität
    • 1
    Monoklonal
    Konjugat
    • 1
    Unkonjugiert
    Applikation
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP)
    Marke
    BD Pharmingen™
    Produktmerkmale
    The 6C1 antibody reacts with common epitopes of PIR-A and PIR-B (Paired Immunoglobulin-liked Receptors) in all mouse strains tested (A/J, BALB/cJ, C3H, C57BL/6, DBA/1, DBA/2, NZB, and SJL). PIR-A and PIR-B are type-I transmembrane glycoproteins containing six Ig-like domains. There are multiple PIR-A proteins which are activating receptors by virtue of their intracellular association with the ITAM (Immunoreceptor Tyrosine-based Activation Motif)- containing Fc receptor γ chain (FcRγ) in mast cells and macrophages. FcRγ expression is required for PIR-A cell-surface expression on dendritic cells, B cells, and myeloid lineages. In contrast, PIR-B is an inhibitory receptor which contains various ITIMs (Immunoreceptor Tyrosine-based Inhibitory Motifs) in its cytoplasmic domain. These receptors are expressed on B cells, granulocytes, mast cells, dendritic cells, and monocytes/macrophages, but not on thymocytes, T lymphocytes, erythroid lineage cells, or NK cells. The level of cell-surface expression of these receptors increases as a function of B-cell activation and myeloid- and B-lineage differentiation. The 6C1 antibody immunoprecipitates molecules of 85- and 125- kDa, which correspond to PIRA and PIR-B, respectively. The expression of PIR-A/B on mouse spleen cells. BALB/c splenocytes were preincubated with Mouse BD Fc Block™ (Cat. no. 553141/553142) and stained with purified 6C1 monoclonal antibody (shaded histogram) followed by biotin-conjugated RG11/39.4 mAb (anti-rat IgG1, Cat. no. 553890, both histograms), then Streptavidin-PE (Cat. no. 554061, both histograms). Flow cytometry was performed on a BD FACScan™

    BD Pharmingen™ Purified Rat Anti-Mouse PIR-A/B - Purified - Clone 6C1 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Recombinant mouse PIR-A1 extracellular domain peptide and the mouse myeloid cell line, WEH13
    Klon
    6C1
    Isotyp
    IgG1 kappa
  • Applikationshinweise
    Flow Cytometry System.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store undiluted at 4°C.
  • Chen, Hurez, Brockenbrough, Kubagawa, Cooper: "Paternal monoallelic expression of the paired immunoglobulin-like receptors PIR-A and PIR-B." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 12, pp. 6868-72, (1999) (PubMed).

    Taylor, McVicar: "Functional association of FcepsilonRIgamma with arginine(632) of paired immunoglobulin-like receptor (PIR)-A3 in murine macrophages." in: Blood, Vol. 94, Issue 5, pp. 1790-6, (1999) (PubMed).

    Kubagawa, Chen, Ho, Shimada, Gartland, Mashburn, Uehara, Ravetch, Cooper: "Biochemical nature and cellular distribution of the paired immunoglobulin-like receptors, PIR-A and PIR-B." in: The Journal of experimental medicine, Vol. 189, Issue 2, pp. 309-18, (1999) (PubMed).

    Bléry, Kubagawa, Chen, Vély, Cooper, Vivier: "The paired Ig-like receptor PIR-B is an inhibitory receptor that recruits the protein-tyrosine phosphatase SHP-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 5, pp. 2446-51, (1998) (PubMed).

    Maeda, Kurosaki, Kurosaki: "Paired immunoglobulin-like receptor (PIR)-A is involved in activating mast cells through its association with Fc receptor gamma chain." in: The Journal of experimental medicine, Vol. 188, Issue 5, pp. 991-5, (1998) (PubMed).

    Kubagawa, Burrows, Cooper: "A novel pair of immunoglobulin-like receptors expressed by B cells and myeloid cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 10, pp. 5261-6, (1997) (PubMed).

  • Target
    PIR-A/B
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