MLH1 Antikörper

Produktnummer ABIN2689847

Produktdetails anti-MLH1 Antikörper

Target: MLH1 (MutL Homolog 1 (MLH1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MLH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Marke: BD Pharmingen™

Produktmerkmale: The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. In bacteria the DNA repair process is accomplished by the MutL, MutH, and MutS proteins. The MutS protein initially recognizes and binds to mismatched DNA. Following this, MutH, an endonuclease, and MutL form a complex with MutS and carry out an excision repair mechanism. When bacteria are deficient in one of these enzymes a mutator phenotype arises characterized by genetic instability. The important role played by DNA repair enzymes is emphasized by the fact that they are highly conserved from bacteria to yeast to mammals. In yeast the proteins are called MutS homolog 2 (MSH2), MutL homolog (MLH1), and PMS1 which is also a homolog of MutL. MSH2 is involved in the initial recognition of mismatched nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMS1 which together help facilitate the later steps in mismatch repair. Biochemical studies of the human homologs of DNA mismatch repair enzymes MLH1, PMS2, and MSH2 indicate that human MSH2 protein can bind mispaired DNA, and that human MLH1 and PMS2 can exist as a heterodimer. These and other studies support the conservation of eukaryotic DNA mismatch repair mechanisms. The G168-15 antibody recognizes human MLH1 (80-85 kDa). Full-length human recombinant MLH was expressed as a fusion protein, affinity purified, and used as immunogen. Immunohistochemistry of MLH-1 in human colon carcinoma. Frozen sections of human colon carcinoma were reacted with the MLH-1 antibody. Cells expressing MLH-1 can be identifed by the intense brown labeling of their cell nuclei. Magnification 20X.

BD Pharmingen™ Purified Mouse Anti-Human MLH-1 - Purified - Clone G168-15 - Isotype Mouse IgG1 - Reactivity Hu - 1 mL

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human recombinant MLH

Klon: G168

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-MLH1 Antikörper (ABIN2689847)

Baker, Plug, Prolla, Bronner, Harris, Yao, Christie, Monell, Arnheim, Bradley, Ashley, Liskay: "Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over." in: Nature genetics, Vol. 13, Issue 3, pp. 336-42, (1996) (PubMed).

Cleaver: "It was a very good year for DNA repair." in: Cell, Vol. 76, Issue 1, pp. 1-4, (1994) (PubMed).

Prolla, Christie, Liskay: "Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene." in: Molecular and cellular biology, Vol. 14, Issue 1, pp. 407-15, (1994) (PubMed).

Prolla, Pang, Alani, Kolodner, Liskay: "MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast." in: Science (New York, N.Y.), Vol. 265, Issue 5175, pp. 1091-3, (1994) (PubMed).

Su, Modrich: "Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 14, pp. 5057-61, (1986) (PubMed).

Details zu MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Andere Bezeichnung: MLH-1 (MLH1 Produkte)

Synonyme: CG11482 antikoerper, Dmel\\CG11482 antikoerper, dmlh-1 antikoerper, dmlh1 antikoerper, zgc:66301 antikoerper, MLH1 antikoerper, LOC100232198 antikoerper, 1110035C23Rik antikoerper, AI317206 antikoerper, AI325952 antikoerper, AI561766 antikoerper, COCA2 antikoerper, FCC2 antikoerper, HNPCC antikoerper, HNPCC2 antikoerper, hMLH1 antikoerper, mutL homolog 1 antikoerper, CG11482 gene product from transcript CG11482-RB antikoerper, mutL homolog 1 S homeolog antikoerper, mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) antikoerper, DNA mismatch repair protein Mlh1 antikoerper, MLH1 antikoerper, Mlh1 antikoerper, mlh1.S antikoerper, mlh1 antikoerper, LOC588545 antikoerper

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response