HLAG Antikörper

Produktnummer ABIN2689559

Produktdetails anti-HLAG Antikörper

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HLAG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: BD Pharmingen™

Produktmerkmale: HLA-G is a HLA class I molecule, but it is distinct from other class I molecules because of its quasi-monomorphism and restricted tissue distribution. It can be expressed as seven distinct protein forms. HLA-G1, -G2, G3, and -G4 are membrane bound while HLA-G5, -G6, and G7 are soluble. HLA-G is expressed in healthy individuals on amniocytes and cytotrophoblasts in the amnion-chorion. HLA-G interacts with inhibitory receptors such as ILT-2, ILT-4, p49 and KIR2DL4 expressed on NK cells, shifts the cytokine balance towards Th2 dominance, suppresses the proliferation of allogeneic CD4 lymphocytes and, in its soluble form, induces apoptosis of activated CD8 cells and inhibits cytolysis by NK cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of human HLA-G (denatured) reactivity on fixed, permeabilized JEG-3 cell line analyzed by flow cytometry. Fixed and permeabilized with Cat. No. 554714. Second step staining with Cat. No. 555988.

BD Pharmingen™ Purified Mouse Anti-human HLA-G Denatured - Purified - Clone 4H84 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: human HLA-G Denatured

Klon: 4H84

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-HLAG Antikörper (ABIN2689559)

Dorling, Monk, Lechler: "HLA-G inhibits the transendothelial migration of human NK cells." in: European journal of immunology, Vol. 30, Issue 2, pp. 586-93, (2000) (PubMed).

King, Allan, Bowen, Powis, Joseph, Verma, Hiby, McMichael, Loke, Braud: "HLA-E is expressed on trophoblast and interacts with CD94/NKG2 receptors on decidual NK cells." in: European journal of immunology, Vol. 30, Issue 6, pp. 1623-31, (2000) (PubMed).

Le Bouteiller, Blaschitz: "The functionality of HLA-G is emerging." in: Immunological reviews, Vol. 167, pp. 233-44, (1999) (PubMed).

Le Bouteiller, Solier, Pröll, Aguerre-Girr, Fournel, Lenfant: "Placental HLA-G protein expression in vivo: where and what for?" in: Human reproduction update, Vol. 5, Issue 3, pp. 223-33, (1999) (PubMed).

Le Gal, Riteau, Sedlik, Khalil-Daher, Menier, Dausset, Guillet, Carosella, Rouas-Freiss: "HLA-G-mediated inhibition of antigen-specific cytotoxic T lymphocytes." in: International immunology, Vol. 11, Issue 8, pp. 1351-6, (1999) (PubMed).

McMaster, Zhou, Shorter, Kapasi, Geraghty, Lim, Fisher: "HLA-G isoforms produced by placental cytotrophoblasts and found in amniotic fluid are due to unusual glycosylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 12, pp. 5922-8, (1998) (PubMed).

Details zu HLAG

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Andere Bezeichnung: HLA-G (HLAG Produkte)

Synonyme: MHC-G antikoerper, B-F antikoerper, B-F-S04 antikoerper, B-F-S05 antikoerper, B-F-S06 antikoerper, B-F-S07 antikoerper, B-FI antikoerper, B-FIV antikoerper, BF2 antikoerper, BFa2 antikoerper, BFw-03 antikoerper, BFw-05 antikoerper, BFz-01 antikoerper, major histocompatibility complex, class I, G antikoerper, MHC BF1 class I antikoerper, HLA-G antikoerper, BF1 antikoerper

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints