Cyclin E1 Antikörper

Produktnummer ABIN2689485

Produktdetails anti-Cyclin E1 Antikörper

Target: Cyclin E1 (CCNE1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin E1 Antikörper ist unkonjugiert

Applikation: Immunoprecipitation (IP)

Marke: BD Pharmingen™

Produktmerkmale: Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small proteins that bind to cyclins, cdks, or cyclin-cdk complexes, e.g., p21 and p27[Kip1]. Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large T-Ag), tumor suppressor genes (e.g., retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. Cyclin E is expressed in G1 and associates with cdk2 to form an active kinase. Cyclin E-cdk2 complexes play an important role in regulation of the G1/S restriction checkpoint in the cell cycle. Aberrant expression of cyclin E is associated with oncogenic transformation of cells. Cyclin E migrates at a reduced molecular weight of ~50 kDa.

BD Pharmingen™ Purified Mouse Anti-Human Cyclin E - Purified - Clone HE67 - Isotype Mouse IgG2b - Reactivity Hu - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant human Cyclin E

Klon: HE67

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-Cyclin E1 Antikörper (ABIN2689485)

Keyomarsi, Herliczek: "The role of cyclin E in cell proliferation, development and cancer." in: Progress in cell cycle research, Vol. 3, pp. 171-91, (1998) (PubMed).

Keyomarsi, OLeary, Molnar, Lees, Fingert, Pardee: "Cyclin E, a potential prognostic marker for breast cancer." in: Cancer research, Vol. 54, Issue 2, pp. 380-5, (1994) (PubMed).

Faha, Harlow, Lees: "The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2." in: Journal of virology, Vol. 67, Issue 5, pp. 2456-65, (1993) (PubMed).

Sherr: "Mammalian G1 cyclins." in: Cell, Vol. 73, Issue 6, pp. 1059-65, (1993) (PubMed).

Details zu Cyclin E1

Target: Cyclin E1 (CCNE1)

Andere Bezeichnung: Cyclin E (CCNE1 Produkte)

Synonyme: CCNE1 antikoerper, ccne antikoerper, cyclinE antikoerper, CCNE antikoerper, AW538188 antikoerper, CycE1 antikoerper, CYCLE antikoerper, Ccne antikoerper, cyce2 antikoerper, cycE3 antikoerper, cyce antikoerper, fa18e07 antikoerper, wu:fa18e07 antikoerper, cyclin E1 antikoerper, cyclin E1 L homeolog antikoerper, cyclin E1 S homeolog antikoerper, CCNE1 antikoerper, ccne1 antikoerper, Ccne1 antikoerper, ccne1.L antikoerper, ccne1.S antikoerper

Molekulargewicht: 50 kDa

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases