CD63 Antikörper
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- Target Alle CD63 Antikörper anzeigen
- CD63
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Reaktivität
- Ratte
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD63 Antikörper ist unkonjugiert
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Applikation
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Marke
- BD Pharmingen™
- Produktmerkmale
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The AD1 antibody reacts with a 50-60- kDa protein that is a homolog (87 % ) of human CD63 (melanomaassociated antigen ME491). CD63, a highly glycosylated type-III lysosomal glycoprotein, is a member of the tetraspan transmembrane 4 superfamily (TM4SF) that also includes CD9, CD37, and CD53. In the rat, CD63 is expressed on mast cells and platelets. CD63 is also found on rat embryonic fibroblasts and hepatic cell, along with cultured cell lines of diverse origin (eg, liver, lung, and heart). Expression is upregulated with culture. CD63 is expressed in close proximity to the FcεRI on rat mast cells and has been shown to partially inhibit IgE-mediated histamine release. The function of CD63 has not been fully characterized. The expression of CD63 on rat CD54+ peritoneal lavage cells. Lewis rat peritoneal lavage cells were incubated simultaneously with either purified Mouse IgG1, κ isotype control (Cat. no. 557273, open histogram) or purified AD1 mAb (shaded histogram), followed by biotinylated A85-1 mAb (rat anti-mouse IgG1, Cat. no. 553441) and finally Streptavidin-FITC (Cat. no. 554060) and PE-conjugated 1A29 mAb (anti-rat CD54, Cat. no. 554970). For analysis, a gate was drawn to include CD54+ cells only (gate not shown). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
BD Pharmingen™ Purified Mouse Anti-Rat CD63 - Purified - Clone AD1 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.1 mg - Aufreinigung
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Rat basophilic leukemia cell line
- Klon
- AD1
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product CD63 Primärantikörper
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store undiluted at 4°C.
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Response of mucosal mast cells to intestinal ischemia-reperfusion injury in the rat." in: Shock (Augusta, Ga.), Vol. 3, Issue 2, pp. 125-31, (1995) (PubMed).
: "The rat mast cell antigen AD1 (homologue to human CD63 or melanoma antigen ME491) is expressed in other cells in culture." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 862-70, (1992) (PubMed).
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Response of mucosal mast cells to intestinal ischemia-reperfusion injury in the rat." in: Shock (Augusta, Ga.), Vol. 3, Issue 2, pp. 125-31, (1995) (PubMed).
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- Target
- CD63
- Andere Bezeichnung
- CD63 (CD63 Produkte)
- Synonyme
- C75951 antikoerper, ME491 antikoerper, Tspan30 antikoerper, LAMP-3 antikoerper, MLA1 antikoerper, OMA81H antikoerper, TSPAN30 antikoerper, TSP29FA antikoerper, CD63 antigen antikoerper, CD63 molecule antikoerper, Cd63 molecule antikoerper, Cd63 antikoerper, CD63 antikoerper
- Hintergrund
- Synonyms: ME491
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