CD34 Antikörper (Biotin)

Produktnummer ABIN2689204

Produktdetails anti-CD34 Antikörper (Biotin)

Target: CD34

Reaktivität: Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD34 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Marke: BD Pharmingen™

Produktmerkmale: The 2E9 antibody reacts with CD34, an ~110 kDa glycoprotein on the surface of bone marrow-derived progenitors of hematopoietic and endothelial cells. In the bone marrow, 1-3 % of cells are stained with 2E9 mAb, whereas peripheral blood leukocytes are not stained. Immunomagnetic depletion of lineage-committed leukocytes from bone marrow results in about three-fold enrichment of CD34+ cells. CD34+ hematopoietic progenitors may be mobilized to the peripheral blood treatment with recombinant canine granulocyte colony-stimulating factor and stem-cell factor. Furthermore, CD34 is expressed on some canine leukemias. In the mouse, CD34 is also expressed on high endothelial venules (HEV) of lymph nodes and, in this form, functions as a ligand for L-selectin. CD34 expression on HEV of dog lymph nodes has been demonstrated with polyclonal anti-CD34 antibody.

BD Pharmingen™ Biotin Mouse Anti-Canine CD34 - Biotin - Clone 2E9 - Isotype Mouse IgG1, κ - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: CD34-mouse IgG2a fusion protein and canine myelomonocytic leukemia ML3

Klon: 2E9

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD34 Antikörper (Biotin) (ABIN2689204)

Georges, Storb, Bruno, Brodie, Thompson, Taranova, Zaucha, Little, Zellmer, Moore, Gooley, Sale, Kiem, Sandmaier, Lyons, Nash: "Engraftment of DLA-haploidentical marrow with ex vivo expanded, retrovirally transduced cytotoxic T lymphocytes." in: Blood, Vol. 98, Issue 12, pp. 3447-55, (2001) (PubMed).

Goerner, Horn, Peterson, Kurre, Storb, Rasko, Kiem: "Sustained multilineage gene persistence and expression in dogs transplanted with CD34(+) marrow cells transduced by RD114-pseudotype oncoretrovirus vectors." in: Blood, Vol. 98, Issue 7, pp. 2065-70, (2001) (PubMed).

Niemeyer, Hudson, Bridgman, Spano, Nash, Lothrop: "Isolation and characterization of canine hematopoietic progenitor cells." in: Experimental hematology, Vol. 29, Issue 6, pp. 686-93, (2001) (PubMed).

Bhattacharya, McSweeney, Shi, Bruno, Ishida, Nash, Storb, Sauvage, Hammond, Wu: "Enhanced endothelialization and microvessel formation in polyester grafts seeded with CD34(+) bone marrow cells." in: Blood, Vol. 95, Issue 2, pp. 581-5, (2000) (PubMed).

Hägglund, McSweeney, Mathioudakis, Bruno, Georges, Gass, Moore, Sale, Storb, Nash: "Ex vivo expansion of canine dendritic cells from CD34+ bone marrow progenitor cells." in: Transplantation, Vol. 70, Issue 10, pp. 1437-42, (2000) (PubMed).

Bruno, Nash, Wallace, Gass, Thompson, Storb, McSweeney: "CD34+ selected bone marrow grafts are radioprotective and establish mixed chimerism in dogs given high dose total body irradiation." in: Transplantation, Vol. 68, Issue 3, pp. 338-44, (1999) (PubMed).

McSweeney, Rouleau, Wallace, Bruno, Andrews, Krizanac-Bengez, Sandmaier, Storb, Wayner, Nash: "Characterization of monoclonal antibodies that recognize canine CD34." in: Blood, Vol. 91, Issue 6, pp. 1977-86, (1998) (PubMed).

McSweeney, Rouleau, Storb, Bolles, Wallace, Beauchamp, Krizanac-Bengez, Moore, Sale, Sandmaier, de Revel, Appelbaum, Nash: "Canine CD34: cloning of the cDNA and evaluation of an antiserum to recombinant protein." in: Blood, Vol. 88, Issue 6, pp. 1992-2003, (1996) (PubMed).

Baumheter, Singer, Henzel, Hemmerich, Renz, Rosen, Lasky: "Binding of L-selectin to the vascular sialomucin CD34." in: Science (New York, N.Y.), Vol. 262, Issue 5132, pp. 436-8, (1993) (PubMed).

Details zu CD34

Target: CD34

Andere Bezeichnung: CD34 (CD34 Produkte)

Synonyme: AU040960 antikoerper, CD34 molecule antikoerper, CD34 antigen antikoerper, CD34 antikoerper, Cd34 antikoerper

Hintergrund: Synonyms: gp 105-120