CD34 Antikörper

Produktnummer ABIN2689202

Produktdetails anti-CD34 Antikörper

Target: CD34

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD34 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Marke: BD Pharmingen™

Produktmerkmale: The RAM34 antibody reacts with the CD34 glycoprotein on the surface of three independently derived mouse CD34-transfected cell lines. RAM34 antibody also reacts with the mouse cell lines PA6, 416B, Swiss 3T6, NIH, 3T3, DA1, and M1, all of which are positive for expression of mouse CD34 mRNA. Cell lines shown to be negative for CD34 transcript, including WEHI-3B, EL4, 18.8, and CMT64/61, are also negative for surface expression of CD34 as determined by RAM34 staining. Normal thymocytes and splenocytes are negative for CD34 expression. In the bone marrow, 7-10 % of cells are stained with RAM34 mAb, including most of the Ly-6A/E (Sca-1)+ CD90 (Thy-1)low Lineage Marker- hematopoietic stem cell-enriched subpopulation and myeloerythroid progenitors. CD34 is also expressed on a small percentage of fetal liver cells, including NK-cell progenitors. CD34 has been reported to be expressed on the endothelium of capillaries and, in this form, to function as a ligand for L-selectin. Consistent with this observation, RAM34 antibody stains endothelial cells in spleen, thymus, and postcapillary HEVs in the lymph nodes. It is reported that RAM34 antibody can be used to select CD34+ CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker- bone marrow-derived hematopoietic stem cells, capable of short-term multi-lineage reconstitution of lethally irradiated mice, while the CD34- CD117+ Sca-1+ Lineage Marker- population contains self-renewing hematopoietic stem cells. Similarly, the bone marrow population with high dye-efflux capacity and which is highly enriched for long-term reconstituting hematopoietic stem cells is CD34- CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker-. Identification of CD34+ and CD34- subpopulations of hematopoietic progenitors. BALB/C mouse bone marrow cells were incubated with either purified monoclonal antibody RAM34 (right panel) or purified rat IgG2a, κ isotype control (clone R35-95, Cat. No. 553927, left panel), followed by second step staining with FITC polyclonal goat-anti-rat Ig (554016). Cells were washed and blocked with mouse Fc Block purified anti-CD16/CD32mAb (553141/553142) and normal rat serum, followed by staining with APC Lineage Cocktail (558074) to identify major lineage committed cells. The contour plots were derived from the gated events based on light scattering characteristics of leukocytes and fluorescence characteristics of the CD34 and Lineage markers . Flow cytometry was performed on a BD FACSCanto™ System. Immunohistochemistry application of CD34 antibody. Immunohistochemistry staining of anti-mouse CD34 antibody clone RAM34 on formalin fixed paraffin embedded mouse embryonic (day 11) tissue section with citrate buffer for antigen retrieval. The antibody stains on endothelial cells of vessels and blood stem cells in vessels (lower panel) with isotype control (upper panel). The secondary antibody was used biotin polyclonal goat anti-rat IgG (559286) and followed by Streptavidin -HRP (550946), and DAB detection kit (550880). Original amplification 40X. 553731 Rev. 1 Page 1 of 2

BD Pharmingen™ Purified Rat Anti-Mouse CD34 - Purified - Clone RAM34 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant Mouse CD34

Klon: RAM34

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CD34 Antikörper (ABIN2689202)

Akashi, Traver, Miyamoto, Weissman: "A clonogenic common myeloid progenitor that gives rise to all myeloid lineages." in: Nature, Vol. 404, Issue 6774, pp. 193-7, (2000) (PubMed).

Lu, Patrene, Herberman, Boggs: "Expression of murine CD34 by fetal liver NK cell progenitors." in: Experimental hematology, Vol. 27, Issue 2, pp. 272-81, (1999) (PubMed).

Goodell, Rosenzweig, Kim, Marks, DeMaria, Paradis, Grupp, Sieff, Mulligan, Johnson: "Dye efflux studies suggest that hematopoietic stem cells expressing low or undetectable levels of CD34 antigen exist in multiple species." in: Nature medicine, Vol. 3, Issue 12, pp. 1337-45, (1998) (PubMed).

Morel, Szilvassy, Travis, Chen, Galy: "Primitive hematopoietic cells in murine bone marrow express the CD34 antigen." in: Blood, Vol. 88, Issue 10, pp. 3774-84, (1996) (PubMed).

Osawa, Hanada, Hamada, Nakauchi: "Long-term lymphohematopoietic reconstitution by a single CD34-low/negative hematopoietic stem cell." in: Science (New York, N.Y.), Vol. 273, Issue 5272, pp. 242-5, (1996) (PubMed).

Suzuki, Andrew, Gonzalo, Fukumoto, Spellberg, Hashiyama, Takimoto, Gerwin, Webb, Molineux, Amakawa, Tada, Wakeham, Brown, McNiece, Ley, Butcher, Suda, Gutierrez-Ramos, Mak: "CD34-deficient mice have reduced eosinophil accumulation after allergen exposure and show a novel crossreactive 90-kD protein." in: Blood, Vol. 87, Issue 9, pp. 3550-62, (1996) (PubMed).

Suda, Sudo, Ito, Ohno, Yamaguchi, Suda: "Two types of murine CD34 mRNA generated by alternative splicing." in: Blood, Vol. 79, Issue 9, pp. 2288-95, (1992) (PubMed).

Brown, Greaves, Molgaard: "The gene encoding the stem cell antigen, CD34, is conserved in mouse and expressed in haemopoietic progenitor cell lines, brain, and embryonic fibroblasts." in: International immunology, Vol. 3, Issue 2, pp. 175-84, (1991) (PubMed).

Spangrude, Heimfeld, Weissman: "Purification and characterization of mouse hematopoietic stem cells." in: Science (New York, N.Y.), Vol. 241, Issue 4861, pp. 58-62, (1988) (PubMed).

Details zu CD34

Target: CD34

Andere Bezeichnung: CD34 (CD34 Produkte)

Synonyme: AU040960 antikoerper, CD34 molecule antikoerper, CD34 antigen antikoerper, CD34 antikoerper, Cd34 antikoerper