CTLA4 Antikörper

Produktnummer ABIN2688988

Produktdetails anti-CTLA4 Antikörper

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CTLA4 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marke: BD Pharmingen™

Produktmerkmale: Reacts with the "cytolytic T lymphocyte-associated antigen", CTLA-4. CTLA-4 is transiently expressed on activated CD28+ T cells and binds to CD80 and CD86 present on antigen presenting cells (APC) with high avidity. This interaction appears to deliver a negative regulatory signal to the T cell. There are recent reports that indicate that CTLA-4 is also expressed on B cells when cultured with activated T cells, suggesting a possible role of CTLA-4 in the regulation of B-cell response. Immobilized BNI3.1 enhances T-cell proliferation induced by CD3 and CD28. Recent studies have showed that CD152 can be expressed by regulatory T (Treg) cells. It has been found this antibody can stain the intracellular CD152 on the Treg cells after fixation and permeabilization of cells. Immunohistochemical staining of CTLA-4 positive cells. Frozen section of normal human tonsil was reacted with the BNI3.1 antibody. Activated T- and B-lymphocytes positive for CTLA-4 can be identified by the intense brown labeling of their cell membranes. Amplification 40X.

BD Pharmingen™ Purified Mouse Anti-Human CD152 - Purified - Clone BNI3 - Isotype Mouse IgG2a, κ - Reactivity Hu - 1 mL

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Klon: BNI3

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-CTLA4 Antikörper (ABIN2688988)

Morton, Fu, Stewart, Giacoletto, White, Leysath, Evans, Shieh, Karr: "Differential effects of CTLA-4 substitutions on the binding of human CD80 (B7-1) and CD86 (B7-2)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 3, pp. 1047-54, (1996) (PubMed).

Kuiper, Brouwer, Linsley, van Lier: "Activated T cells can induce high levels of CTLA-4 expression on B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 4, pp. 1776-83, (1995) (PubMed).

Lindsten, Lee, Harris, Petryniak, Craighead, Reynolds, Lombard, Freeman, Nadler, Gray: "Characterization of CTLA-4 structure and expression on human T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 7, pp. 3489-99, (1993) (PubMed).

Details zu CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Andere Bezeichnung: CD152 (CTLA4 Produkte)

Synonyme: CTLA-4 antikoerper, CTLA4 antikoerper, CD152 antikoerper, Cd152 antikoerper, Ctla-4 antikoerper, Ly-56 antikoerper, CD antikoerper, CELIAC3 antikoerper, GRD4 antikoerper, GSE antikoerper, IDDM12 antikoerper, sCTLA4 antikoerper, cytotoxic T-lymphocyte associated protein 4 antikoerper, cytotoxic T-lymphocyte-associated protein 4 antikoerper, CTLA4 antikoerper, Ctla4 antikoerper

Hintergrund: Synonyms: CTLA-4

Pathways: Cancer Immune Checkpoints