Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

TNFRSF1B Antikörper

TNFRSF1B Reaktivität: Human FACS Wirt: Ratte Monoclonal hTNFR unconjugated
Produktnummer ABIN2688926
  • Target Alle TNFRSF1B Antikörper anzeigen
    TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
    Reaktivität
    • 86
    • 62
    • 34
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Human
    Wirt
    • 62
    • 31
    • 12
    • 5
    • 4
    • 1
    • 1
    Ratte
    Klonalität
    • 61
    • 55
    Monoklonal
    Konjugat
    • 68
    • 19
    • 9
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser TNFRSF1B Antikörper ist unkonjugiert
    Applikation
    • 69
    • 43
    • 33
    • 33
    • 32
    • 24
    • 19
    • 17
    • 14
    • 9
    • 7
    • 6
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Marke
    BD Pharmingen™
    Produktmerkmale
    The hTNFR-M1 antibody reacts with the extracellular domain of the 75 kDa transmembrane receptor for the human cytokines, tumor necrosis factor (TNF or TNF-α) and lymphotoxin-alpha (LT-α3, aka, lymphotoxin or TNF-β). This receptor is referred to as the p75 or Type II Tumor Necrosis Factor Receptor (TNFRII) [aka, CD120b]. Human TNFRII proteins are expressed by hematopoietic cells including macrophages, neutrophils, lymphocytes, thymocytes and mast cells. TNFRII is expressed by a variety of other cell types including endothelial cells, cardiac myocytes and prostate cells. Naive B cells express very low or undetectable levels of TNFRII whereas mature erythrocytes and platelets are uniformly negative for TNFRII expression. The immunogen used to generate the hTNFR-M1 hybridoma was COS- expressed recombinant human TNFRII. Expression of cell surface TNFRII by whole-lysed human blood. Whole human blood was lysed with PharmLyse™ (Cat. No. 555899) prior to staining with hTNFR-M1. Whole lysed human blood was subsequently blocked with normal polyclonal human IgG and stained with purified hTNFR-M1 (0.06 μg/10e6 cells, Cat No. 551311) followed by biotinylated goat anti-rat F(ab')2 IgG and streptavidin phycoerythrin (0.015 μg, Cat. No. 554061). Staining with the hTNFR-M1 antibody (filled histograms) is compared to staining obtained using the isotype control antibody (open histograms). The histograms in the figure were derived from gated events with the light scattering characteristics of viable lymphocytes (left panel), monocytes (center panel) and granulocytes (right panel). Note: Certain human cell lines or cell types (e.g., neutrophils, monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.

    BD Pharmingen™ Purified Rat Anti-Human CD120b - Purified - Clone hTNFR-M1 - Isotype Rat IgG2b, κ - Reactivity Hu - 0.5 mg
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    COS-expressed recombinant human TNFRII
    Klon
    HTNFR
    Isotyp
    IgG2b kappa
    Top Product
    Discover our top product TNFRSF1B Primärantikörper
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store undiluted at 4°C.
  • Aggarwal, Gollapudi, Gupta: "Increased TNF-alpha-induced apoptosis in lymphocytes from aged humans: changes in TNF-alpha receptor expression and activation of caspases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 4, pp. 2154-61, (1999) (PubMed).

    Browning, Dougas, Ngam-ek, Bourdon, Ehrenfels, Miatkowski, Zafari, Yampaglia, Lawton, Meier: "Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 33-46, (1995) (PubMed).

    Gehr, Gentz, Brockhaus, Loetscher, Lesslauer: "Both tumor necrosis factor receptor types mediate proliferative signals in human mononuclear cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 911-7, (1992) (PubMed).

    Wallach, Engelmann, Nophar, Aderka, Kemper, Hornik, Holtmann, Brakebusch: "Soluble and cell surface receptors for tumor necrosis factor." in: Agents and actions. Supplements, Vol. 35, pp. 51-7, (1992) (PubMed).

    Heilig, Mapara, Brockhaus, Krauth, Dörken: "Two types of TNF receptors are expressed on human normal and malignant B lymphocytes." in: Clinical immunology and immunopathology, Vol. 61, Issue 2 Pt 1, pp. 260-7, (1991) (PubMed).

    Brockhaus, Schoenfeld, Schlaeger, Hunziker, Lesslauer, Loetscher: "Identification of two types of tumor necrosis factor receptors on human cell lines by monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 8, pp. 3127-31, (1990) (PubMed).

    Hohmann, Remy, Brockhaus, van Loon: "Two different cell types have different major receptors for human tumor necrosis factor (TNF alpha)." in: The Journal of biological chemistry, Vol. 264, Issue 25, pp. 14927-34, (1989) (PubMed).

    Munker, DiPersio, Koeffler: "Tumor necrosis factor: receptors on hematopoietic cells." in: Blood, Vol. 70, Issue 6, pp. 1730-4, (1988) (PubMed).

  • Target
    TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
    Andere Bezeichnung
    CD120b (TNFRSF1B Produkte)
    Synonyme
    CD120b antikoerper, TBPII antikoerper, TNF-R-II antikoerper, TNF-R75 antikoerper, TNFBR antikoerper, TNFR1B antikoerper, TNFR2 antikoerper, TNFR80 antikoerper, p75 antikoerper, p75TNFR antikoerper, TNF-RII/TNFRSF1B antikoerper, TNFR-II antikoerper, TNFRSF1B antikoerper, TNF-R2 antikoerper, TNF-alphaR2 antikoerper, TNFRII antikoerper, TNFalpha-R2 antikoerper, Tnfr-1 antikoerper, Tnfr2 antikoerper, rank antikoerper, zgc:163064 antikoerper, TNF receptor superfamily member 1B antikoerper, tumor necrosis factor receptor superfamily, member 1b antikoerper, tumor necrosis factor receptor superfamily, member 1B antikoerper, TNFRSF1B antikoerper, Tnfrsf1b antikoerper, tnfrsf1b antikoerper
    Hintergrund
    Synonyms: TNF Receptor Type II
    Pathways
    NF-kappaB Signalweg, Apoptose, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway
Sie sind hier:
Kundenservice