Androgen Receptor Antikörper

Produktnummer ABIN2688843

Produktdetails anti-Androgen Receptor Antikörper

Target: Androgen Receptor (AR)

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Androgen Receptor Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marke: BD Pharmingen™

Produktmerkmale: The androgen receptor (AR) mediating androgen effects is a member of the steroid and thyroid hormone receptor gene superfamily encoding ligand-dependent nuclear transcription factors. They can be divided into three main domains, an N-terminal domain which modulates transcription efficiency, a central DNA domain which binds to a target gene hormone response element, and a C-terminal hormone binding domain. Androgens are necessary for normal male development and function. They exert their effects on target tissue through binding to the AR, followed by association of the AR complex with specific binding sites on DNA. This AR complex may induce or repress gene transcription. Mutations in the AR gene are associated with androgen insensitivity syndrome, a disorder that causes XY genotypic males to develop as phenotypic females because of their inability to respond to androgens. AR mutations have also been identified in human prostate cancer specimens and the LNCaP human prostate cancer cell line. Monoclonal and polyclonal antibodies to the AR have identified AR-positive cells in a variety of tissues including male and female sexual organs, kidney, liver, adrenal cortex, pituitary gland, skeletal, cardiac, and smooth muscle cells. The androgen receptor migrates at a molecular weight of ~100 kD in SDS-PAGE. G122-25 recognizes an epitope localized within the DNA-binding domain (between amino acids 486 and 651) of the human androgen receptor. Additionally, G122-25 has been shown to recognize androgen receptors in frozen rat prostate tissue sections. G122-25 does not cross-react with estrogen or progesterone receptors. Purified recombinant human androgen receptor protein was used as immunogen.

BD Pharmingen™ Purified Mouse Anti-Human Androgen Receptor - Purified - Clone G122-25 - Isotype Mouse IgG1 - Reactivity Hu, Rat - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified human androgen receptor recombinant protein

Klon: G122

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-Androgen Receptor Antikörper (ABIN2688843)

Fuller: "The steroid receptor superfamily: mechanisms of diversity." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 5, Issue 15, pp. 3092-9, (1992) (PubMed).

Newmark, Hardy, Tonb, Carter, Epstein, Isaacs, Brown, Barrack: "Androgen receptor gene mutations in human prostate cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 14, pp. 6319-23, (1992) (PubMed).

Details zu Androgen Receptor

Target: Androgen Receptor (AR)

Andere Bezeichnung: Androgen Receptor (AR Produkte)

Synonyme: AIS antikoerper, DHTR antikoerper, HUMARA antikoerper, HYSP1 antikoerper, KD antikoerper, NR3C4 antikoerper, SBMA antikoerper, SMAX1 antikoerper, TFM antikoerper, AW320017 antikoerper, Tfm antikoerper, Andr antikoerper, AR antikoerper, androgen receptor antikoerper, AR antikoerper, Ar antikoerper, ar antikoerper

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding