Histone 3 Antikörper (H3K4me3)
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- Target Alle Histone 3 (H3) Antikörper anzeigen
- Histone 3 (H3)
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Bindungsspezifität
- H3K4me3
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Reaktivität
- Human, Saccharomyces cerevisiae
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Histone 3 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
- Aufreinigung
- Protein A Chromatography
- Immunogen
- This Histone H3 trimethylLys4 antibody was raised against a peptide including trimethyllysine 4 of histone H3.
- Isotyp
- IgG
- Top Product
- Discover our top product H3 Primärantikörper
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- Applikationshinweise
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Recommended starting concentrations are
ChIP: 3 µg per ChIP
ChIP-Seq: 4 µg each
ICC/IF: 1 - 2 µg/mL dilution
WB: 2 - 4 µg/mL dilution
CUT&RUN: 1:100
Optimal working dilution should be determined by the investigator. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1 μg/μL
- Buffer
- Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
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Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Antibodies in solution can be stored at -20 °C for 2 years.
- Haltbarkeit
- 6 months
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H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).
: "Reduced expression of SET7/9, a histone mono-methyltransferase, is associated with gastric cancer progression." in: Oncotarget, Vol. 7, Issue 4, pp. 3966-83, (2016) (PubMed).
: "Alterations in nuclear structure promote lupus autoimmunity in a mouse model." in: Disease models & mechanisms, Vol. 9, Issue 8, pp. 885-97, (2016) (PubMed).
: "The long noncoding RNA HOTAIR has tissue and cell type-dependent effects on HOX gene expression and phenotype of urothelial cancer cells." in: Molecular cancer, Vol. 14, pp. 108, (2015) (PubMed).
: "A balancing act: heterochromatin protein 1a and the Polycomb group coordinate their levels to silence chromatin in Drosophila." in: Epigenetics & chromatin, Vol. 8, pp. 17, (2015) (PubMed).
: "Sex Specification and Heterogeneity of Primordial Germ Cells in Mice." in: PLoS ONE, Vol. 10, Issue 12, pp. e0144836, (2015) (PubMed).
: "Loss of histone H3 methylation at lysine 4 triggers apoptosis in Saccharomyces cerevisiae." in: PLoS genetics, Vol. 10, Issue 1, pp. e1004095, (2014) (PubMed).
: "The 3D tissue microenvironment modulates DNA methylation and E-cadherin expression in squamous cell carcinoma." in: Epigenetics, Vol. 7, Issue 1, pp. 34-46, (2012) (PubMed).
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H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).
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- Target
- Histone 3 (H3)
- Andere Bezeichnung
- Histone H3 (H3 Produkte)
- Synonyme
- H-3 antikoerper, histocompatibility 3 antikoerper, H3 antikoerper
- Molekulargewicht
- 17 kDa
- Gen-ID
- 3020
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