PDPK1 Antikörper (3-phosphoinositide Dependent Protein Kinase-1) (C-Term)

Details for Product anti-PDPK1 Antibody No. ABIN250776, Anbieter: Anmelden zum Anzeigen
Antigen
  • pdpk1
  • MGC82080
  • PDK1
  • PDPK2
  • PRO0461
  • Pdk1
  • ATP5A
  • si:zfos-979f1.3
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • 3'-phosphoinositide-dependent protein kinase 1
  • 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1
  • ATPDK1
  • AtPDK1
  • T32M21.110
  • T32M21_110
  • zgc:77318
  • 3-phosphoinositide dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase 1 L homeolog
  • 3-phosphoinositide dependent protein kinase-1
  • ATP synthase, H+ transporting, mitochondrial Fo complex subunit C2 (subunit 9)
  • 3-phosphoinositide-dependent protein kinase 1
  • pyruvate dehydrogenase kinase, isozyme 1
  • pyruvate dehydrogenase kinase 1
  • Phosphoinositide-dependent kinase 1
  • 3'-phosphoinositide-dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase 1b
  • PDPK1
  • pdpk1.L
  • pdpk1
  • Pdpk1
  • ATP5G2
  • LOC100380750
  • pdk1
  • PDK1
  • Pdk1
  • pdk-1
  • pdpk1b
Epitop
C-Term, AA 350-436
58
30
28
27
26
17
15
15
15
12
12
8
7
7
6
6
6
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
446
243
236
21
13
12
11
9
6
5
4
3
2
1
1
1
1
1
1
Wirt
Kaninchen
387
54
16
Klonalität
Polyklonal
Konjugat
Dieser PDPK1 Antikörper ist unkonjugiert
17
15
14
10
10
7
7
7
7
7
7
7
7
7
4
4
3
3
2
2
2
2
2
2
2
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
307
158
131
91
65
48
27
25
18
11
10
6
3
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen A synthetic peptide made to a C-terminal portion of the human PDK1 protein sequence (between residues 350-436). [Swiss-Prot Q15118]
Produktmerkmale PDK1 (Pyruvate Dehydrogenase Kinase Isoenzyme 1) plays a role in glucose and metabolism of fatty acid regulation via phosphorylation of the pyruvate dehydrogenase subunits. PDK1 also inhibits the activity of pyruvate dehydrogenase and helps regulate metabolite change. PDK1 cellular response to hypoxia is important for cell proliferation under hypoxic conditions. PDK1 also protects against apoptosis in response to oxidative stress and conditions of low oxygen.
Reinigung Immunogen affinity purified
Plasmids, Primers & others Plasmide, Primers & weitere PDPK1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung PDK-1 (PDPK1 Antibody Abstract)
Hintergrund Gene Symbol: PDK1
Molekulargewicht Theoretical MW: 49 kDa
Gen-ID 5163
UniProt Q15118
Pathways PI3K-Akt Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
Applikations-hinweise Western Blot 2 μg/mL, Immunocytochemistry/Immunofluorescence 1:1000-1:2000This PDK1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot. By Western blot a band is seen at ~49 kDa, representing PDK1 protein. There are also two higher and one lower non-specific molecular weight bands. In ICC/IF, mitochondiral staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for PDK1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 1 hour at room temperature (RT).
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-PDK1 primary antibody in blocking buffer and incubate 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (we used BioFX Super Plus ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.42 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung -80 °C,-20 °C
Informationen zur Lagerung Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Bilder des Herstellers
Western Blotting (WB) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 350-436), (C-Term) antibody (ABIN250776) Detection of PDK1 in human heart lysate using ABIN250776 at 2 ug/ml.
Western Blotting (WB) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 350-436), (C-Term) antibody (ABIN250776) Western Blot: PDK-1 Antibody [ABIN250776] - Analysis of HeLa lysates using ABIN250776...
Immunofluorescence (IF) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 350-436), (C-Term) antibody (ABIN250776) Immunocytochemistry/Immunofluorescence: PDK-1 Antibody [ABIN250776] - Antibody was te...
Produkt verwendet in: Tsoi, Li, Chen, Lau, Tsui, Chan: "The SARS-coronavirus membrane protein induces apoptosis via interfering with PDK1-PKB/Akt signalling." in: The Biochemical journal, Vol. 464, Issue 3, pp. 439-47, 2014 (PubMed). (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Immunofluorescence

Luo, Chang, Zhong, Pandey, Semenza: "Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 49, pp. E3367-76, 2012 (PubMed).

Luo, Hu, Chang, Zhong, Knabel, OMeally, Cole, Pandey, Semenza: "Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1." in: Cell, Vol. 145, Issue 5, pp. 732-44, 2011 (PubMed).

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