COL2A1 Antikörper (Chain alpha 1)

Produktnummer ABIN113965

Produktdetails anti-COL2A1 Antikörper

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Bindungsspezifität: Chain alpha 1

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COL2A1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Spezifität: Murine Procollagen and Collagen type II: 100 % . Murine Collagen type I, III and IV < 0.1 %. Mouse Fibronectin: < 0.1 %. Cross-reacts with Bovine Collagen type II in ELISA.

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Aufreinigung: Affinity Chromatography.

Immunogen: Purified Collagen type II from Fetal Murine Cartilage.

Anwendungsinformationen

Applikationshinweise: ELISA: 1/200 (OD >/= 500). Radioimmunoassay. Immunofluorescence: 1/80. Immunohistochemistry (Parrafin): 1/1000 (60 min at RT or 2-8 °C over night). Positive Control: Mouse Cartilage. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water, for further dilution use appropriate antibody diluent.

Konzentration: 1.0 mg/mL

Buffer: PBS without BSA and preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu COL2A1

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Abstract: COL2A1 Produkte

Synonyme: ANFH antikoerper, AOM antikoerper, COL11A3 antikoerper, SEDC antikoerper, Col2 antikoerper, Col2a antikoerper, Col2a-1 antikoerper, Del1 antikoerper, Dmm antikoerper, Lpk antikoerper, M100856 antikoerper, Rgsc856 antikoerper, CG2A1A antikoerper, COLLII antikoerper, col2a1a antikoerper, CB11 antikoerper, col2a1 antikoerper, coll2a1 antikoerper, fb38c06 antikoerper, fc10c01 antikoerper, wu:fb38c06 antikoerper, wu:fc10c01 antikoerper, collagen type II alpha 1 chain antikoerper, collagen, type II, alpha 1 antikoerper, collagen, type II, alpha 1 L homeolog antikoerper, collagen, type II, alpha 1 S homeolog antikoerper, collagen, type II, alpha 1a antikoerper, COL2A1 antikoerper, Col2a1 antikoerper, col2a1 antikoerper, col2a1.L antikoerper, col2a1.S antikoerper, col2a1a antikoerper

Hintergrund: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type II collagen is an alpha1(II)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and various tumours.Synonyms: Alpha-1 type II collagen, COL2A1

Gen-ID: 12824

NCBI Accession: NP_001106987

UniProt: P28481

Pathways: Sensory Perception of Sound, Growth Factor Binding