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PUMA Antikörper (C-Term) (Atto 594)

BBC3 Reaktivität: Human WB, ICC, IF Wirt: Kaninchen Polyclonal Atto 594
Produktnummer ABIN2485947
  • Target Alle PUMA (BBC3) Antikörper anzeigen
    PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
    Bindungsspezifität
    • 20
    • 18
    • 16
    • 10
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 113
    • 40
    • 39
    • 16
    Human
    Wirt
    • 109
    • 7
    • 1
    Kaninchen
    Klonalität
    • 98
    • 19
    Polyklonal
    Konjugat
    • 53
    • 8
    • 8
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser PUMA Antikörper ist konjugiert mit Atto 594
    Applikation
    • 98
    • 40
    • 39
    • 22
    • 22
    • 17
    • 15
    • 14
    • 13
    • 9
    • 5
    • 3
    • 2
    • 2
    Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Spezifität
    Detects ~23 kDa. Detects ~16 kDa bands sometimes, possibly corresponding to PUMAβ.
    Kreuzreaktivität
    Huhn, Human, Maus
    Aufreinigung
    Protein A Purified
    Immunogen
    C-terminal amino acids of human PUMA
    Top Product
    Discover our top product BBC3 Primärantikörper
  • Applikationshinweise
    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    1 μg/ml of ABIN2485947 was sufficient for detection of PUMA in 20 μg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS, 0.02 % sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Conjugated antibodies should be stored at 4°C
  • Target
    PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
    Andere Bezeichnung
    PUMA (BBC3 Produkte)
    Synonyme
    BBC3 antikoerper, Puma antikoerper, JFY-1 antikoerper, JFY1 antikoerper, PUMA antikoerper, PUMA/JFY1 antikoerper, BCL2 binding component 3 antikoerper, Bcl-2 binding component 3 antikoerper, BBC3 antikoerper, Bbc3 antikoerper, bbc3 antikoerper
    Hintergrund
    Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
    Gen-ID
    27113
    NCBI Accession
    NP_001120712
    UniProt
    Q9BXH1
    Pathways
    p53 Signalweg, Positive Regulation of Endopeptidase Activity
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