Fusion protein containing 73 aas from region aa800-900 (according to NP_079511.1). No crossreactivity expected with TANK1.
Aufreinigung
TNKS2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TNKS2 antibody was raised against a 12 amino acid synthetic peptide near the internal of TNKS2.
TNKS2
Reaktivität: Human
ELISA
Wirt: Kaninchen
Polyclonal
Biotin
Applikationshinweise
Western Blot: In transfected HEK293 transiently expressing TANK2 a band of approximately 127 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 127 kDa according to NP_079511.1. Recommended concentration, 0.5-1.5 μg/mL. Full image caption: HEK293 overexpressing TANK2 (lane 4) and TANK1 (lane 2) and probed with 42-018 (mock transfection in first lane). Lane three is empty. Lower panel shows the same lysates probed for alpha-Actin to show protein levels. Primary incubation (0.5 μg/mL) was overnight at 4 °C. Detected by chemiluminescence.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.