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BCKDHA Antikörper

BCKDHA Reaktivität: Human, Maus, Ratte WB, ELISA Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN2463953
  • Target Alle BCKDHA Antikörper anzeigen
    BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))
    Reaktivität
    • 23
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 20
    • 3
    Kaninchen
    Klonalität
    • 22
    • 1
    Polyklonal
    Konjugat
    • 19
    • 2
    • 1
    • 1
    Dieser BCKDHA Antikörper ist unkonjugiert
    Applikation
    • 17
    • 7
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA
    Aufreinigung
    Antibody is purified by peptide affinity chromatography method.
    Immunogen
    Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BCKDHA.
    Top Product
    Discover our top product BCKDHA Primärantikörper
  • Applikationshinweise
    BCKDHA antibody can be used for detection of BCKDHA by ELISA at 1:312500. BCKDHA antibody can be used for detection of BCKDHA by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
    Konzentration
    1 mg/mL
    Buffer
    Antibody is lyophilized in PBS buffer with 2 % sucrose.
    Handhabung
    As with any antibody avoid repeat freeze-thaw cycles.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    For short periods of storage (days) store at 4 °C. For longer periods of storage, store BCKDHA antibody at -20 °C.
  • Target
    BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))
    Andere Bezeichnung
    BCKDHA (BCKDHA Produkte)
    Synonyme
    wu:fd20d04 antikoerper, zgc:110049 antikoerper, BCKDE1A antikoerper, MSU antikoerper, MSUD1 antikoerper, OVD1A antikoerper, BCKDA antikoerper, E1a antikoerper, 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial antikoerper, branched chain keto acid dehydrogenase E1, alpha polypeptide antikoerper, 2-oxoisovalerate dehydrogenase alpha subunit (branched-chain alpha-keto acid dehydrogenase E1 component alpha chain) (BCKDH E1-alpha) (BCKDE1A) antikoerper, branched chain ketoacid dehydrogenase E1, alpha polypeptide antikoerper, LOC704978 antikoerper, bckdha antikoerper, BCKDHA antikoerper, BckdhA antikoerper, Bckdha antikoerper
    Hintergrund
    The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD, EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine. This reaction is the second major step in the catabolism of the branched-chain amino acids (Wynn et al., 1998 [PubMed 9582350]). The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2, MIM 248610), and a homodimeric dihydrolipoamide dehydrogenase (E3, MIM 238331). E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene (MIM 248611) encodes the beta subunit of E1. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.
    Molekulargewicht
    50 kDa
    Gen-ID
    593
    NCBI Accession
    NP_000700
    UniProt
    P12694
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