GNAI1
Reaktivität: Human
ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
GNAI1 antibody can be used for detection of GNAI1 by ELISA at 1:12500. GNAI1 antibody can be used for detection of GNAI1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Konzentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handhabung
As with any antibody avoid repeat freeze-thaw cycles.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
For short periods of storage (days) store at 4 °C. For longer periods of storage, store GNAI1 antibody at -20 °C.
AU046200 antikoerper, Gialpha1 antikoerper, Gnai-1 antikoerper, gnai1 antikoerper, Gi antikoerper, BPGTPB antikoerper, zgc:63957 antikoerper, guanine nucleotide binding protein (G protein), alpha inhibiting 1 antikoerper, G protein subunit alpha i1 antikoerper, guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 S homeolog antikoerper, guanine nucleotide-binding protein G(i) subunit alpha-1 antikoerper, guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 antikoerper, Gnai1 antikoerper, gnai1.S antikoerper, GNAI1 antikoerper, LOC397505 antikoerper, gnai1 antikoerper
Hintergrund
Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase, Go, a protein abundant in brain (GNAO1), and transducin-1 (GNAT1) and transducin-2 (GNAT2), proteins involved in phototransduction in retinal rods and cones, respectively.Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase, Go, a protein abundant in brain (GNAO1, MIM 139311), and transducin-1 (GNAT1, MIM 139330) and transducin-2 (GNAT2, MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218], Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein, see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM]. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.