RFT1 Antikörper

Produktnummer ABIN2459371

Produktdetails anti-RFT1 Antikörper

Target: RFT1 (RFT1 Homolog (RFT1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFT1.

Anwendungsinformationen

Applikationshinweise: RFT1 antibody can be used for detection of RFT1 by ELISA at 1:1562500. RFT1 antibody can be used for detection of RFT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RFT1 antibody at -20 °C.

Details zu RFT1

Target: RFT1 (RFT1 Homolog (RFT1))

Andere Bezeichnung: RFT1 (RFT1 Produkte)

Synonyme: GB14370 antikoerper, CDG1N antikoerper, D930025H04 antikoerper, RGD1562654 antikoerper, protein RFT1 homolog antikoerper, RFT1 homolog antikoerper, LOC412489 antikoerper, LOC100453537 antikoerper, RFT1 antikoerper, rft1 antikoerper, LOC100633251 antikoerper, LOC100651701 antikoerper, Rft1 antikoerper

Hintergrund: N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a Man (5)GlcNAc (2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man (5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation.N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a Man (5)GlcNAc (2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man (5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation (Helenius et al., 2002 [PubMed 11807558]).

Molekulargewicht: 60 kDa

Gen-ID: 91869

NCBI Accession: NP_443091

UniProt: Q96AA3