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Rhp51 Antikörper

RHP51 Reaktivität: Schizosaccharomyces pombe IF, IP, ELISA, WB Wirt: Kaninchen Polyclonal unconjugated
Pubmed (3 Referenzen)
Produktnummer ABIN2452110
$532.31
Zzgl. Versandkosten $45.00
100 μL
Lieferung in 9 bis 11 Werktagen
  • Target
    Rhp51 (RHP51) (RecA Family Recombinase Rhp51 (RHP51))
    Reaktivität
    Schizosaccharomyces pombe
    Wirt
    Kaninchen
    Klonalität
    Polyklonal
    Applikation
    Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
    Produktmerkmale
    Antiserum
    Aufreinigung
    Rabbit serum
  • Applikationshinweise
    1) Western blotting: 1/ 2,000~1/5,000 dilution
    Kommentare

    This antibody was successfully used for immunoprecipitation assay.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    0.09 % sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt aliquot and store at -20 C
  • Haruta, Kurokawa, Murayama, Akamatsu, Unzai, Tsutsui, Iwasaki: "The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro." in: Nature structural & molecular biology, Vol. 13, Issue 9, pp. 823-30, (2006) (PubMed).

    Lambert, Watson, Sheedy, Martin, Carr: "Gross chromosomal rearrangements and elevated recombination at an inducible site-specific replication fork barrier." in: Cell, Vol. 121, Issue 5, pp. 689-702, (2005) (PubMed).

    Morishita, Furukawa, Sakaguchi, Toda, Carr, Iwasaki, Shinagawa: "Role of the Schizosaccharomyces pombe F-Box DNA helicase in processing recombination intermediates." in: Molecular and cellular biology, Vol. 25, Issue 18, pp. 8074-83, (2005) (PubMed).

  • Target
    Rhp51 (RHP51) (RecA Family Recombinase Rhp51 (RHP51))
    Andere Bezeichnung
    Rhp51
    Synonyme
    RecA family recombinase Rhp51 antikoerper, rhp51 antikoerper
    Hintergrund
    Rhp51 protein of Schizosaccharomyces pombe (fission yeast) is a functional and structural homolog of E.coli RecA protein and Rad51 proteins of eukaryotes, which play a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands. The product was prepared by immunizing rabbit with full-size recombinant Rhp51 protein which was expressed in E. coli. and then purified. Using this anti-serum, Rhp51 protein (40 kD) in the crude extract was detected by Western blotting and Rhp51 foci formation induced by stalled replication and DNA-damage was detected by indirect immunofluorescence. GFP-tagging of Rhp51 protein at either N- or C- terminus inactivates the function of Rhp51, and therefore, the GFP-tagged antibody cannot be used for the study of foci formation.
    UniProt
    P36601
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