Rhp51 Antikörper
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- Target
- Rhp51 (RHP51) (RecA Family Recombinase Rhp51 (RHP51))
- Reaktivität
- Schizosaccharomyces pombe
- Wirt
- Kaninchen
- Klonalität
- Polyklonal
- Applikation
- Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
- Produktmerkmale
- Antiserum
- Aufreinigung
- Rabbit serum
-
- Applikationshinweise
- 1) Western blotting: 1/ 2,000~1/5,000 dilution
- Kommentare
-
This antibody was successfully used for immunoprecipitation assay.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Buffer
- 0.09 % sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Upon receipt aliquot and store at -20 C
-
-
The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro." in: Nature structural & molecular biology, Vol. 13, Issue 9, pp. 823-30, (2006) (PubMed).
: "Gross chromosomal rearrangements and elevated recombination at an inducible site-specific replication fork barrier." in: Cell, Vol. 121, Issue 5, pp. 689-702, (2005) (PubMed).
: "Role of the Schizosaccharomyces pombe F-Box DNA helicase in processing recombination intermediates." in: Molecular and cellular biology, Vol. 25, Issue 18, pp. 8074-83, (2005) (PubMed).
: "
-
The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro." in: Nature structural & molecular biology, Vol. 13, Issue 9, pp. 823-30, (2006) (PubMed).
-
- Target
- Rhp51 (RHP51) (RecA Family Recombinase Rhp51 (RHP51))
- Andere Bezeichnung
- Rhp51
- Synonyme
- RecA family recombinase Rhp51 antikoerper, rhp51 antikoerper
- Hintergrund
- Rhp51 protein of Schizosaccharomyces pombe (fission yeast) is a functional and structural homolog of E.coli RecA protein and Rad51 proteins of eukaryotes, which play a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands. The product was prepared by immunizing rabbit with full-size recombinant Rhp51 protein which was expressed in E. coli. and then purified. Using this anti-serum, Rhp51 protein (40 kD) in the crude extract was detected by Western blotting and Rhp51 foci formation induced by stalled replication and DNA-damage was detected by indirect immunofluorescence. GFP-tagging of Rhp51 protein at either N- or C- terminus inactivates the function of Rhp51, and therefore, the GFP-tagged antibody cannot be used for the study of foci formation.
- UniProt
- P36601