ABCB5 Antikörper (Internal Region)

Produktnummer ABIN238622

Der Ziege Polyklonal Anti-ABCB5-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, ABCB5 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für ABCB5 Antikörper (Internal Region) (ABIN238622)

Target: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ABCB5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-ABCB5 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: ABCB5

Sequenz: QTQHRNTSKK AQ

Spezifität: This antibody is expected to recognise both reported isoforms (NP_848654.3, NP_001157413.1)

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QTQHRNTSKKAQ , from the internal region of the protein sequence according to NP_848654.3, NP_001157413.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 80 kDa band observed in lysates of cell line A431(calculated MW of 89.8 kDa according to NP_848654.3). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-ABCB5 Antikörper (ABIN238622)

Cheung, Cheung, Cheng, Wong, Fan: "Granulin-epithelin precursor and ATP-dependent binding cassette (ABC)B5 regulate liver cancer cell chemoresistance." in: Gastroenterology, Vol. 140, Issue 1, pp. 344-55, (2010) (PubMed).

Details zu ABCB5

Target: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Andere Bezeichnung: ABCB5

Hintergrund: ATP-binding cassette, sub-family B (MDR/TAP), member 5 , ABCB5alpha, ABCB5beta, EST422562, ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5

Molekulargewicht: 89.8kDa

Gen-ID: 340273

NCBI Accession: NP_848654, NP_001157413