His Tag Antikörper

Details zu Produkt Nr. ABIN223514, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
624
28
15
4
4
1
Wirt
Kaninchen
414
187
27
23
8
8
3
2
Klonalität
Polyklonal
Konjugat
Unkonjugiert
58
46
37
17
13
13
10
10
9
9
9
9
8
8
8
7
7
7
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Immunochromatography (IC), Immunoprecipitation (IP), Western Blotting (WB)
568
406
164
108
83
82
78
27
12
11
8
7
6
6
5
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen 6xHis synthetic peptide
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN412778.
Sequenz HHHHHH
Isotyp IgG
Spezifität The antibody detects His-tagged recombinant proteins.
Reinigung Affinity purified
Andere Bezeichnung His-Tag
Substanzklasse Tag
Hintergrund A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
Synonyms: His tag, HHHHHH tag
Applikationshinweise Western blotting (1 μg/mL), immunoprecipitation (40 μg/mL), and immunocytochemistry (2 μg/mL). However, the optimal conditions should be determined individually. The antibody detects His-tagged recombinant proteins.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) peptide affinity purified rabbit anti-His-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.
Konservierungsmittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung The antibody solution should be gently mixed before use.
Lagerung -20 °C
Informationen zur Lagerung For long-term storage, aliquot and freeze at -70 °C.
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for anti-His Tag antibody (ABIN223514) anti-His Tag antibody
Produkt verwendet in: Yin, Johns, Lawrence, Xu, Reddi, Bishop, Varner, Fuster: "Lymphatic endothelial heparan sulfate deficiency results in altered growth responses to vascular endothelial growth factor-C (VEGF-C)." in: The Journal of biological chemistry, Vol. 286, Issue 17, pp. 14952-62, 2011 (PubMed).

Tsang, Felek, Krukonis: "Ail binding to fibronectin facilitates Yersinia pestis binding to host cells and Yop delivery." in: Infection and immunity, Vol. 78, Issue 8, pp. 3358-68, 2010 (PubMed).

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