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Apoptotic Neutrophils Antikörper (FITC)

Reaktivität: Human FACS, WB Wirt: Maus Monoclonal BOB93 FITC
Pubmed (1 reference)
Produktnummer ABIN2192015
$885.41
Zzgl. Versandkosten $45.00
100 μg
Lieferung in 6 bis 8 Werktagen
  • Target See all Apoptotic Neutrophils products
    Apoptotic Neutrophils
    Reaktivität
    Human
    Wirt
    • 4
    Maus
    Klonalität
    • 4
    Monoklonal
    Konjugat
    • 3
    • 1
    Dieser Apoptotic Neutrophils Antikörper ist konjugiert mit FITC
    Applikation
    Flow Cytometry (FACS), Western Blotting (WB)
    Sterilität
    0.2 μm filtered
    Klon
    BOB93
  • Applikationshinweise
    For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    PBS, containing 1 % bovine serum albumin and 0.02 % sodium azide.
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
    Haltbarkeit
    12 months
  • Hart, Jackson, Kremmel, McNeill, Jersmann, Alexander, Ross, Dransfield: "Specific binding of an antigen-antibody complex to apoptotic human neutrophils." in: The American journal of pathology, Vol. 162, Issue 3, pp. 1011-8, (2003) (PubMed).

  • Target
    Apoptotic Neutrophils
    Abstract
    Apoptotic Neutrophils Produkte
    Hintergrund
    The monoclonal antibody BOB93 binds to the surface of apoptotic neutrophils. Neutrophils have been implicated in the pathogenenesis of a variety of inflammatory diseases including the adult respiratory distress syndrome, idiopathic pulmonary fibrosis, ulcerative colitis and rheumatoid arthritis. Although the neutrophil is a vital component of the body's defense against infectious agents, uncontrolled release of its toxic substances may inflict "friendly fire" damage on surrounding tissue and propagate the inflammatory response, leading to scarring and tissue destruction. Apoptosis leads to recognition and safe disposal of dying cells by phagocytosis. Binding of the monoclonal antibody BOB93 to apoptotic neutrophils is dependent on the presence of sialoglycoprotein fetuin, a constituent of bovine serum. Fetuin is the antigen for BOB93, and BOB93 and fetuin form a complex in solution that is necessary and sufficient for binding to apoptotic neutrophils. The antigen recognised by BOB93 may act as a "molecular bridge" between the surface of the apoptotic neutrophil and the phagocyte to modulate apoptotic neutrophil clearance at inflammatory sites. Human macrophage phagocytosis of apoptotic neutrophils was augmented in vitro by addition of the antigen recognised by BOB93, which does not bind to other apoptotic leukocytes.
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