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CKB Antikörper (Biotin)

CKB Reaktivität: Human IHC (fro), IHC (p), WB Wirt: Maus Monoclonal CK-BYK-21E10 Biotin
Pubmed (2 Referenzen)
Produktnummer ABIN2191989
$430.52
Zzgl. Versandkosten $45.00
50 μg
Lieferung in 6 bis 8 Werktagen
  • Target Alle CKB Antikörper anzeigen
    CKB
    Reaktivität
    • 117
    • 54
    • 36
    • 12
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 92
    • 35
    • 8
    Maus
    Klonalität
    • 92
    • 42
    Monoklonal
    Konjugat
    • 61
    • 22
    • 12
    • 7
    • 7
    • 7
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Dieser CKB Antikörper ist konjugiert mit Biotin
    Applikation
    • 95
    • 68
    • 27
    • 18
    • 16
    • 12
    • 10
    • 10
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Kreuzreaktivität (Details)
    Cross reactivity: Rabbit : Yes, Mouse : Yes, Rat : Yes
    Sterilität
    0.2 μm filtered
    Klon
    CK-BYK-21E10
    Isotyp
    IgG2b
  • Applikationshinweise
    For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Human Purkinje cells control Negative Human fibroblasts control
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
  • Sistermans, de Kok, Peters, Ginsel, Jap, Wieringa: "Tissue- and cell-specific distribution of creatine kinase B: a new and highly specific monoclonal antibody for use in immunohistochemistry." in: Cell and tissue research, Vol. 280, Issue 2, pp. 435-46, (1995) (PubMed).

    de Kok, Geurds, Sistermans, Usmany, Vlak, Wieringa: "Production of native creatine kinase B in insect cells using a baculovirus expression vector." in: Molecular and cellular biochemistry, Vol. 143, Issue 1, pp. 59-65, (1995) (PubMed).

  • Target
    CKB
    Andere Bezeichnung
    Creatine Kinase B-Type (CKB Produkte)
    Synonyme
    B-CK, CKBB, b-ck, ckbb, CKB, Bck, Ck-3, Ck3, Ckbb, bck, ckb, cb594, wu:fj36f10, wu:fj37d11, Ckbr, creatine kinase B, creatine kinase, brain L homeolog, creatine kinase B-type, creatine kinase, brain, creatine kinase, brain b, CKB, ckb.L, Ckb, ckb, ckbb
    Hintergrund
    The monoclonal antibody CK-BYK/21E10 recognizes human creatine kinase B-type, also known as CKB. Human CKB is a protein of 381 amino acids (~45 kDa), expressed in a number of tissues. CKB is most abundant in adult brain, approx. 5-fold lower in the stomach, 10-fold lower in the heart and barely detectable in liver. In brain, whereas most CKB has been shown to be cytosolic, several of the reactions requiring CKB are membrane-associated. CKB belongs to the creatine kinase (CK) isoenzymes that catalyse the synthesis of phosphocreatine (PCr) and its subsequent use in the regeneration of ATP in cell types where the consumption of ATP is rapid and/or sudden. In the brain the different CK isoforms constitute an energy shuttle wherein ATP produced in the mitochondria is used by a mitochondrial CK [e.g. ubiquitous mitochondrial creatine kinase (uMi-CK)] to generate PCr, which is then transported and used by a cytoplasmic CK [e.g. brain creatine kinase (CKB)] to regenerate ATP at discrete cellular sites of high ATP turnover. CKB appears to have a role in regenerating ATP needed for the transport of ions and neurotransmitters since CKB has been localized to brain synaptic plasma membranes, possibly coupled to Na+/K+-ATPase and acetylcholine receptor-rich membranes. Expression of CKB is developmentally controlled: in rat, brain CKB protein at birth is extremely low and increases 10-fold until week 4. This reflects the many energy-demanding processes in brain during brain development. Aliases Creatine kinase B chain, B-CK, BB-CK Immunogen Synthetic 17-mer peptide corresponding to the N-terminal sequence of human creatine kinase brain- type (SNSHNALKLRFPAEDEF)
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