TLR3 Antikörper (Toll-Like Receptor 3)

Details for Product anti-TLR3 Antibody No. ABIN2191972
Antigen
  • CD283
  • IIAE2
  • AI957183
  • zgc:123006
  • toll like receptor 3
  • toll-like receptor 3
  • TLR3
  • Tlr3
  • tlr3
Reaktivität
Human
207
145
57
8
2
1
1
1
Wirt
Maus
197
70
9
2
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser TLR3 Antikörper ist unkonjugiert
30
27
17
9
8
6
3
3
3
3
3
3
1
1
1
1
1
Applikation
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
178
88
84
66
30
27
24
20
13
11
10
7
5
4
1
1
Optionen
Klon TLR3-7
Isotyp IgG1
Kreuzreaktivität (Details) Cross reactivity: 4 Canine TLR3 : Yes, Mouse TLR3 : Yes
Sterilität 0.2 μm filtered
Plasmids, Primers & others Plasmide, Primers & weitere TLR3 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung Toll-Like Receptor 3 (TLR3 Antibody Abstract)
Hintergrund The monoclonal antibody TLR3.7 recognizes the 116 kDa human Toll-like receptor 3 (TLR3, CD283). Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called 'pathogen-associated molecular patterns'. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFκB- dependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single α-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses. Some forms of RNA and DNA from pathogens exhibit immutable features that distinguish them from nucleic acids of higher organisms. For example, dsRNA, is a common intermediate of viral replication and a potent indicator of infection. Toll-like receptor 3 (TLR3) recognizes viral double-stranded RNA and its synthetic analog polyriboinosinic:polyribocytidylic acid (poly(I:C)). TLR3 is normally located in acidic endosomes where its luminal ectodomain (ECD) encounters dsRNA and induces type I interferon (IFN), inflammatory cytokine/chemokine production and dendritic cell (DC) maturation via the adaptor protein TICAM-1 (also called TRIF). Based on the different subcellular localization of cytosolic RNA receptors and TLR3, these receptors seem to play distinct roles in anti-viral immune responses. Aliases CD283 Immunogen Human Flag-tagged TLR3 stably expressed by Ba/F3 cells
Pathways TLR Signalweg, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
Applikations-hinweise For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells control Negative HEK293 control
Beschränkungen Nur für Forschungszwecke einsetzbar
Buffer PBS, containing 0.1 % bovine serum albumin.
Lagerung 4 °C
Informationen zur Lagerung Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Produkt verwendet in: Burgener, Jungi: "Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry." in: Veterinary immunology and immunopathology, Vol. 124, Issue 1-2, pp. 184-91, 2008 (PubMed).

Schreiner, Voss, Wischhusen, Dombrowski, Steinle, Lochmüller, Dalakas, Melms, Wiendl: "Expression of toll-like receptors by human muscle cells in vitro and in vivo: TLR3 is highly expressed in inflammatory and HIV myopathies, mediates IL-8 release and up-regulation of NKG2D-ligands." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 1, pp. 118-20, 2006 (PubMed).

Jorgenson, Young, Lesmeister, Lyddon, Misfeldt: "Human endometrial epithelial cells cyclically express Toll-like receptor 3 (TLR3) and exhibit TLR3-dependent responses to dsRNA." in: Human immunology, Vol. 66, Issue 5, pp. 469-82, 2005 (PubMed).

Oshiumi, Matsumoto, Funami, Akazawa, Seya: "TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction." in: Nature immunology, Vol. 4, Issue 2, pp. 161-7, 2003 (PubMed).

Matsumoto, Funami, Tanabe, Oshiumi, Shingai, Seto, Yamamoto, Seya: "Subcellular localization of Toll-like receptor 3 in human dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 6, pp. 3154-62, 2003 (PubMed).

Matsumoto, Kikkawa, Kohase, Miyake, Seya: "Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling." in: Biochemical and biophysical research communications, Vol. 293, Issue 5, pp. 1364-9, 2002 (PubMed).

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