Red Blood Cell Antikörper

Produktnummer ABIN2191860

Produktdetails anti-Red Blood Cell Antikörper

Target: Red Blood Cell (RBC)

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Red Blood Cell Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Functional Studies (Func)

Kreuzreaktivität (Details): Cross reactivity: Rat, sheep, rabit, chicken, human : No

Sterilität: 0.2 μm filtered

Klon: 34-3C

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Mouse erythrocytes control

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.1 % bovine serum albumin.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Referenzen für anti-Red Blood Cell Antikörper (ABIN2191860)

Baudino, Nimmerjahn, Azeredo da Silveira, Martinez-Soria, Saito, Carroll, Ravetch, Verbeek, Izui: "Differential contribution of three activating IgG Fc receptors (FcgammaRI, FcgammaRIII, and FcgammaRIV) to IgG2a- and IgG2b-induced autoimmune hemolytic anemia in mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 3, pp. 1948-53, (2008) (PubMed).

Fossati-Jimack, Azeredo da Silveira, Moll, Kina, Kuypers, Oldenborg, Reininger, Izui: "Selective increase of autoimmune epitope expression on aged erythrocytes in mice: implications in anti-erythrocyte autoimmune responses." in: Journal of autoimmunity, Vol. 18, Issue 1, pp. 17-25, (2002) (PubMed).

Azeredo da Silveira, Kikuchi, Fossati-Jimack, Moll, Saito, Verbeek, Botto, Walport, Carroll, Izui: "Complement activation selectively potentiates the pathogenicity of the IgG2b and IgG3 isotypes of a high affinity anti-erythrocyte autoantibody." in: The Journal of experimental medicine, Vol. 195, Issue 6, pp. 665-72, (2002) (PubMed).

Shibata, Berney, Reininger, Chicheportiche, Ozaki, Shirai, Izui: "Monoclonal anti-erythrocyte autoantibodies derived from NZB mice cause autoimmune hemolytic anemia by two distinct pathogenic mechanisms." in: International immunology, Vol. 2, Issue 12, pp. 1133-41, (1991) (PubMed).

Details zu Red Blood Cell

Target: Red Blood Cell (RBC)

Andere Bezeichnung: Rbc (RBC Produkte)

Hintergrund: The monoclonal antibody 34-3C recognizes an exposed surface determinant of intact red blood cells (RBC). The high-affinity anti-RBC monoclonal antibody efficiently bind to Fc receptors on macrophages inducing anemia in vivo due to a rapid Fc receptor (FcyR)-mediated erythrophagocytosis of opsonised RBC in spleen and livers. The capacity of the antibody to interact with FcyR is responsible for its haemolytic activity. The monoclonal antibody only recognises antigenic determinants expressed on Mouse RBC and not on other species of RBC. Immunogen RBC