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CD80 Antikörper (FITC)

CD80 Reaktivität: Human FACS Wirt: Maus Monoclonal 4A2D10-4 FITC
Produktnummer ABIN2144693
  • Target Alle CD80 Antikörper anzeigen
    CD80
    Reaktivität
    • 139
    • 123
    • 44
    • 8
    • 3
    • 3
    • 1
    • 1
    • 1
    Human
    Wirt
    • 88
    • 71
    • 26
    • 24
    • 23
    • 1
    • 1
    Maus
    Klonalität
    • 154
    • 77
    • 2
    Monoklonal
    Konjugat
    • 90
    • 20
    • 19
    • 17
    • 15
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD80 Antikörper ist konjugiert mit FITC
    Applikation
    • 141
    • 94
    • 70
    • 41
    • 34
    • 22
    • 14
    • 14
    • 13
    • 13
    • 12
    • 11
    • 11
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Produktmerkmale
    CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.
    Klon
    4A2D10-4
    Isotyp
    IgG1
    Top Product
    Discover our top product CD80 Primärantikörper
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdurchführung
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
  • Target
    CD80
    Andere Bezeichnung
    CD80 (CD80 Produkte)
    Synonyme
    B7 antikoerper, B7-1 antikoerper, B7.1 antikoerper, BB1 antikoerper, CD28LG antikoerper, CD28LG1 antikoerper, LAB7 antikoerper, B71 antikoerper, Cd28l antikoerper, Ly-53 antikoerper, Ly53 antikoerper, MIC17 antikoerper, TSA1 antikoerper, CD80 molecule antikoerper, Cd80 molecule antikoerper, CD80 antigen antikoerper, CD80 antikoerper, Cd80 antikoerper
    Pathways
    T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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