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Integrin beta 2 Antikörper (FITC)

ITGB2 Reaktivität: Human FACS Wirt: Maus Monoclonal 4ATS1-18-1-2-11 FITC
Produktnummer ABIN2144524
  • Target Alle Integrin beta 2 (ITGB2) Antikörper anzeigen
    Integrin beta 2 (ITGB2)
    Reaktivität
    • 197
    • 53
    • 45
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 101
    • 97
    • 34
    • 2
    Maus
    Klonalität
    • 136
    • 93
    • 2
    Monoklonal
    Konjugat
    • 96
    • 25
    • 21
    • 19
    • 11
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Integrin beta 2 Antikörper ist konjugiert mit FITC
    Applikation
    • 129
    • 87
    • 52
    • 52
    • 39
    • 31
    • 29
    • 26
    • 26
    • 26
    • 21
    • 13
    • 13
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Produktmerkmale
    4ATS1/18.1.2.11 reacts with CD18, the 95 kDa beta chain component of leukocyte function associated antigen-1 (LFA-1). CD18 is expressed by all peripheral blood leukocytes. CD18 is a leukocyte adhesion receptor that is essential for cell-to-cell contact in many immune responses such as lymphocyte adhesion, NK and T cell cytolysis, and T cell proliferation.
    Klon
    4ATS1-18-1-2-11
    Isotyp
    IgG1
    Top Product
    Discover our top product ITGB2 Primärantikörper
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdurchführung
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
  • Target
    Integrin beta 2 (ITGB2)
    Andere Bezeichnung
    CD18 (ITGB2 Produkte)
    Synonyme
    CD18 antikoerper, LAD antikoerper, LCAMB antikoerper, LFA-1 antikoerper, MAC-1 antikoerper, MF17 antikoerper, MFI7 antikoerper, 2E6 antikoerper, AI528527 antikoerper, Cd18 antikoerper, Lfa1 antikoerper, ITGB2 antikoerper, itgb2 antikoerper, itgb2-a antikoerper, itgb2-b antikoerper, integrin subunit beta 2 antikoerper, integrin beta 2 antikoerper, integrin subunit beta 2 S homeolog antikoerper, ITGB2 antikoerper, Itgb2 antikoerper, itgb2.S antikoerper
    Pathways
    NF-kappaB Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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