Human Cells anti-Human IgG2 (AA 99-327), (Ser257Ala-Mutant) Antikörper

Details zu Produkt Nr. ABIN2001827, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
AA 99-327, Ser257Ala-Mutant
68
10
9
8
4
3
3
2
2
1
1
1
Reaktivität
Human
156
33
20
20
17
1
1
1
1
Wirt
Human Cells
143
37
35
30
4
1
Antikörpertyp
Recombinant Antibody
Biologische Aktivität
Active
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Fragment Fc fragment
Produktmerkmale The recombinant human IgG2-Fc consists of 228 amino acids and has a predicted molecular mass of 25 kDa. As a result of glycosylation, the apparent molecular mass of IgG2-Fc is approximately 34 kDa in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human IgG2 Fc region (P01859) (Glu 99-Lys 327, 257Ser/Ala) was expressed and purified.
Predicted N-Term: Glu 99
Reinheit > 90 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to bind human CD32a-His in a functional ELISA.
Antigen IgG2
Hintergrund Synonyms: IgG2
Molekulargewicht 25 kDa, 34 kDa
UniProt P01859
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
SDS-PAGE (SDS) image for Human Cells anti-Human IgG2 (AA 99-327), (Ser257Ala-Mutant) antibody (ABIN2001827) Human Cells anti-Human IgG2 (AA 99-327), (Ser257Ala-Mutant) antibody
Allgemeine Veröffentlichungen Warmerdam, van de Winkel, Vlug, Westerdaal, Capel: "A single amino acid in the second Ig-like domain of the human Fc gamma receptor II is critical for human IgG2 binding." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 4, pp. 1338-43, 1991

Warmerdam, van de Winkel, Vlug, Westerdaal, Capel: "A single amino acid in the second Ig-like domain of the human Fc gamma receptor II is critical for human IgG2 binding." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 4, pp. 1338-43, 1991 (PubMed).