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UBA1 Antikörper (Ubiquitin-Like Modifier Activating Enzyme 1) (AA 2-1058) Primary Antibody

UBA1 Reaktivität: Human ELISA, IP, WB Wirt: Maus Monoclonal 05 unconjugated
Produktnummer ABIN1999473
$285.00
Zzgl. Versandkosten $45.00
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Lieferung in 11 bis 13 Werktagen
  • Target
    UBA1
    Bindungsspezifität
    AA 2-1058
    • 16
    • 14
    • 8
    • 7
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Reaktivität
    Human
    • 75
    • 39
    • 31
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Wirt
    Maus
    • 73
    • 2
    Klonalität
    Monoklonal
    • 65
    • 10
    Konjugat
    Dieser UBA1 Antikörper ist unkonjugiert
    • 25
    • 8
    • 6
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Applikation
    ELISA, Immunoprecipitation (IP), Western Blotting (WB)
    • 53
    • 39
    • 38
    • 13
    • 9
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    Spezifität
    Human UBE1 / UBA1
    No cross-reactivity in ELISA with
    Insect cell lysate
    Keine Kreuzreaktivität
    Insekten
    Produktmerkmale
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human UBE1 / UBA1 (rh UBE1 / UBA1, ABIN2004962 NP_003325.2, Ser2-Arg1058,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
    Aufreinigung
    purified by Protein A affinity chromatography
    Sterilität
    0.2 μm filtered
    Immunogen
    Recombinant Human UBE1 / UBA1 protein (ABIN2004962)
    Klon
    05
    Isotyp
    IgG1
  • Applikationshinweise
    WB: 5-20 μg/mL
    ELISA: 0.5-1 μg/mL
    This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human UBE1 / UBA1. The detection limit for Human UBE1 / UBA1 is approximately 0.039 ng/well.
    IP: 1-4 μL/mg of lysate
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    0.2 μm filtered solution in PBS with 5 % trehalose
    Konservierungsmittel
    Without preservative
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    4 °C,-20 °C,-80 °C
    Informationen zur Lagerung
    This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    UBA1
    Andere Bezeichnung
    UBA1 (UBA1 Antibody Abstract)
    Synonyme
    A1S9, A1S9T, A1ST, AMCX1, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X, DDBDRAFT_0190927, DDBDRAFT_0220497, DDB_0190927, DDB_0220497, Sbx, Ube-1, Ube1x, a1s9, a1s9t, a1st, amcx1, gxp1, poc20, smax2, uba1a, ube1, ube1x, uba1, uba1b, ube, zgc:66143, wu:fa01e08, wu:fb30f01, wu:fi21c11, wu:fj14g11, ubiquitin like modifier activating enzyme 1, ubiquitin activating enzyme E1, Ubiquitin-conjugating enzyme E1, ubiquitin-like modifier activating enzyme 1, ubiquitin-like modifier activating enzyme 1 S homeolog, ubiquitin-like modifier activating enzyme 1 L homeolog, UBA1, uae1, GL50803_4083, GL50803_10661, Uba1, uba1.S, uba1.L, uba1, ptr3
    Hintergrund
    UBE1, also known as UBA1, belongs to the ubiquitin-activating E1 family. It catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It also catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. UBE1 gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Defects in UBA1 are the cause of spinal muscular atrophy X-linked type 2 (SMAX2), also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures.
    NCBI Accession
    NP_003325.2
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