His Tag Antikörper

Details zu Produkt Nr. ABIN1981363, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
624
28
15
4
4
1
Wirt
Maus
413
188
27
23
8
8
3
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
58
46
37
17
13
13
10
10
9
9
9
9
8
8
8
7
7
7
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
568
405
165
108
83
82
78
27
13
11
8
7
6
6
5
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck Monoclonal Antibody to detect His-taaged proteins
Immunogen 6X-HIS-KLH
Sequenz HHHHHH
Klon 11E12
Isotyp IgG1 kappa
Spezifität Monoclonal Antibody detects His-taaged proteins
Produktmerkmale Monoclonal Antibody to detect His-taaged proteins
Reinigung Protein A chromatography from in vitro production
Andere Bezeichnung His-Tag
Substanzklasse Tag
Hintergrund A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
Synonyms: His-Tag
Applikationshinweise ELISA, WB
Kommentare

Suggested positive control:
Western blot: His-Tagged protein

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer 1 mg/mL in 0.15 M PBS
Handhabung The antibody solution should be gently mixed before use.
Lagerung -20 °C
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for anti-His Tag antibody (ABIN1981363) anti-His Tag antibody
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