NFKBIA Antikörper (Ser32, Ser36)

Produktnummer ABIN197304

Produktdetails anti-NFKBIA Antikörper

Target: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Bindungsspezifität: Ser32, Ser36

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFKBIA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: IkappaB-alpha antibody detects endogenous levels of total IkappaB-alpha protein.

Aufreinigung: Immunoaffinity chromatography

Immunogen: Synthetic non-phosphopeptide derived from human IkappaB-alpha around the phosphorylation site of serine 32/36 (H-D-Sp-G-L-D-Sp-M-K).

Anwendungsinformationen

Applikationshinweise: Suitable for use in Western blot (1: 500-1: 1000) and Immunohistochemistry (1: 50-1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Referenzen für anti-NFKBIA Antikörper (ABIN197304)

Tsolou, Liousia, Kalamida, Pouliliou, Giatromanolaki, Koukourakis: "Inhibition of IKK-NFκB pathway sensitizes lung cancer cell lines to radiation." in: Cancer biology & medicine, Vol. 14, Issue 3, pp. 293-301, (2017) (PubMed).

Details zu NFKBIA

Target: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Andere Bezeichnung: NFKBIA / IKBA (NFKBIA Produkte)

Synonyme: IKBA antikoerper, MAD-3 antikoerper, NFKBI antikoerper, AI462015 antikoerper, Nfkbi antikoerper, RL/IF-1 antikoerper, ECI-6/IKBA antikoerper, NFKBIA antikoerper, ikbaB antikoerper, wu:fb81f08 antikoerper, zgc:56666 antikoerper, zgc:77616 antikoerper, ikbaA antikoerper, wu:fj16a07 antikoerper, zgc:85769 antikoerper, NFKB inhibitor alpha antikoerper, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha antikoerper, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha b antikoerper, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha a antikoerper, NFKBIA antikoerper, Nfkbia antikoerper, nfkbiab antikoerper, nfkbiaa antikoerper

Hintergrund: Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IkappaB alpha and IkappaB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkappaB and block nuclear import by masking the nuclear localisation sequences of NFkappaB. The activation of NFkappaB involves the inducible phosphorylation and subsequent degradation of IkappaB. Immunoblotting easily detects the hyperphosphorylated forms of IkappaB alpha, but not phosphorylated IkappaB beta. Interestingly, IkappaB alpha and IkappaB beta mediate different NFkappaB responses. IkappaB alpha appears to control more transient activation of NFkappaB in response to an inducer, while IkappaB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkappaB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IkappaB is confirmed by immunoblotting.Synonyms: I kappa B-alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI

Gen-ID: 4792

NCBI Accession: NP_065390

UniProt: P25963

Pathways: NF-kappaB Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling