GCLC Antikörper

Produktnummer ABIN190704

Produktdetails anti-GCLC Antikörper

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reaktivität: Arabidopsis thaliana, Nicotiana tabacum, Tomato, Mais (Zea mays)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GCLC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunolocalization (IL)

Spezifität: The antibody recognizes closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa)

Kreuzreaktivität (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Homologie: dicots including:Pisum sativum, Riciuns communis, monocots including: Oryza sativa, Triticum aestivum

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 50 kDa
This antibody can be used as a marker of vacuolar contents.

Aufreinigung: affinity purified

Immunogen: KLH-conjugated synthetic peptide derived from Zea mays gamma-ECS Q8W4W3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 1: 5000 with standard ECL (WB), 1: 5000 (IL)

Kommentare:

antibodies has been used in immunolocalization on Arabidopsis thaliana

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water.

Buffer: PBS pH 7.4

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Referenzen für anti-GCLC Antikörper (ABIN190704)

Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).

Keefe, Hinz, Meins: "The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leaves." in: Planta, Vol. 182, Issue 1, pp. 43-51, (2013) (PubMed).

Details zu GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Andere Bezeichnung: Gamma-ECS (GCLC Produkte)

Synonyme: GCLC antikoerper, gclc antikoerper, MGC146287 antikoerper, 2259 antikoerper, CG2259 antikoerper, DmGCLC antikoerper, DmGCS antikoerper, DmGCSh antikoerper, Dmel\\CG2259 antikoerper, GCL antikoerper, GCLc antikoerper, GCSh antikoerper, Gcs antikoerper, Gcsh antikoerper, DDBDRAFT_0186120 antikoerper, DDBDRAFT_0231403 antikoerper, DDB_0186120 antikoerper, DDB_0231403 antikoerper, GCS antikoerper, GLCL antikoerper, GLCLC antikoerper, D9Wsu168e antikoerper, GLCL-H antikoerper, Ggcs-hs antikoerper, Glclc antikoerper, cb1049 antikoerper, fe36e11 antikoerper, wu:fe36e11 antikoerper, glutamate-cysteine ligase catalytic subunit antikoerper, glutamate-cysteine ligase, catalytic subunit antikoerper, Glutamate-cysteine ligase catalytic subunit antikoerper, gamma glutamylcysteine synthetase antikoerper, glutamate-cysteine ligase, catalytic subunit L homeolog antikoerper, glutamate-cysteine ligase, catalytic subunit S homeolog antikoerper, gamma-glutamylcysteine synthetase antikoerper, GCLC antikoerper, gclc antikoerper, Gclc antikoerper, LbGCS antikoerper, gcsA antikoerper, gclc.L antikoerper, gclc.S antikoerper, GSH1 antikoerper

Hintergrund: AGI Code: At4g23100
Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development.

Molekulargewicht: 50 kDa

UniProt: Q8W4W3

Pathways: Cell RedoxHomeostasis