This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This POLR2J3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-25 amino acids from the N-terminal region of human POLR2J3.
Klon
RB45111
Isotyp
Ig Fraction
Applikationshinweise
WB: 1:1000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Haltbarkeit
6 months
Grandemange, Schaller, Yamano, Du Manoir, Shpakovski, Mattei, Kedinger, Vigneron: "A human RNA polymerase II subunit is encoded by a recently generated multigene family." in: BMC molecular biology, Vol. 2, pp. 14, (2003) (PubMed).
Hillier, Fulton, Fulton, Graves, Pepin, Wagner-McPherson, Layman, Maas, Jaeger, Walker, Wylie, Sekhon, Becker, OLaughlin, Schaller, Fewell, Delehaunty, Miner, Nash, Cordes, Du, Sun, Edwards et al.: "The DNA sequence of human chromosome 7. ..." in: Nature, Vol. 424, Issue 6945, pp. 157-64, (2003) (PubMed).
Target
POLR2J3
(Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft (By similarity).