TIE1 Antikörper (AA 701-898)

Produktnummer ABIN1871554

Produktdetails anti-TIE1 Antikörper

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Bindungsspezifität: AA 701-898

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TIE1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Polyclonal Antibody to Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)

Spezifität: The antibody is a rabbit polyclonal antibody raised against Tie1. It has been selected for its ability to recognize Tie1 in immunohistochemical staining and western blotting.

Kreuzreaktivität: Maus, Schwein, Ratte

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1) corresdonding to Thr701~Pro898 (Accession # P35590)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu TIE1

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Andere Bezeichnung: Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (TIE1 Produkte)

Synonyme: JTK14 antikoerper, TIE antikoerper, D430008P04Rik antikoerper, tie-1 antikoerper, tyrosine kinase with immunoglobulin like and EGF like domains 1 antikoerper, tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antikoerper, tyrosine-protein kinase receptor Tie-1 antikoerper, endothelium-specific receptor tyrosine kinase 1 antikoerper, TIE1 antikoerper, Tie1 antikoerper, CpipJ_CPIJ018933 antikoerper, tie1 antikoerper

Hintergrund: JTK14, Tyrosine Kinase With Immunoglobulin And Epidermal Growth Factor Homology Domains 1, Tyrosine-protein kinase receptor Tie-1

Pathways: RTK Signalweg