COL8A1 Antikörper (AA 590-744)

Produktnummer ABIN1858463

Produktdetails anti-COL8A1 Antikörper

Target: COL8A1 (Collagen, Type VIII, alpha 1 (COL8A1))

Bindungsspezifität: AA 590-744

Reaktivität: Kaninchen

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser COL8A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Sequenz: MGHHHHHHSG SEF-L GIDGVKTPHA YAAKKGKNGG PAYEMPAFTA ELTAPFPPVG APIKFDRLLY NGRQNYNPQT GIFTCEVPGV YYFAYHVHCK GGNVWVALFK NNEPVMYTYD EYKKGFLDQA SGSAVLLLRP GDRVFLQMPS EQAAGLYAGQ YVHSSFSGYL LYPM

Spezifität: It has been selected for its ability to recognize COL8a1 in immunohistochemical staining and Western blotting.

Aufreinigung: Affinity Chromatography

Immunogen: Recombinant COL8a1 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against COL8a1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Kommentare:

Content: The quality control contains recombinant COL8a1 (Leu590~Met744) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2% SDS, 200 mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Haltbarkeit: 12 months

Details zu COL8A1

Target: COL8A1 (Collagen, Type VIII, alpha 1 (COL8A1))

Andere Bezeichnung: Collagen Type VIII Alpha 1 (COL8A1 Produkte)

Synonyme: COL8A1 antikoerper, Col8a-1 antikoerper, C3orf7 antikoerper, collagen type VIII alpha 1 chain antikoerper, collagen, type VIII, alpha 1 S homeolog antikoerper, collagen, type VIII, alpha 1 antikoerper, COL8A1 antikoerper, col8a1.S antikoerper, Col8a1 antikoerper