Caspase 3 Antikörper (AA 29-175) (FITC)

Produktnummer ABIN1858244

Produktdetails anti-Caspase 3 Antikörper (FITC)

Target: Caspase 3 (CASP3)

Bindungsspezifität: AA 29-175

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 3 Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Sequenz: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SG IYLDSSYKMD YPEMGLCIII NNKNFHKSTGMSARNGTDVD AANLRETFMA LKYEVRNKND LTREEIMELM DSVSKEDHSK RSSFVCVILS HGDEGVIFGT NGPVDLKKLT SFFRGDYCRS LTGKPKLFII QACRGTELDC GIETD

Spezifität: It has been selected for its ability to recognize CASP3 in immunohistochemical staining and Western blotting.

Aufreinigung: Affinity Chromatography

Immunogen: Recombinant CASP3 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against CASP3 conjugated to fitc.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Kommentare:

Content: The quality control contains recombinant CASP3 (Ser29~Asp175) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Haltbarkeit: 12 months

Referenzen für anti-Caspase 3 Antikörper (FITC) (ABIN1858244)

Chen, Cao, Asare, Lv, Zhu, Li, Wei, Gao, Zhang, Mao, Gao, Fan: "Amelioration of cardiac dysfunction and ventricular remodeling after myocardial infarction by danhong injection are critically contributed by anti-TGF-β-mediated fibrosis and angiogenesis mechanisms." in: Journal of ethnopharmacology, Vol. 194, pp. 559-570, (2016) (PubMed).

Details zu Caspase 3

Target: Caspase 3 (CASP3)

Andere Bezeichnung: Caspase 3 (CASP3 Produkte)

Synonyme: CPP32 antikoerper, CPP32B antikoerper, SCA-1 antikoerper, A830040C14Rik antikoerper, AC-3 antikoerper, Apopain antikoerper, CC3 antikoerper, Caspase-3 antikoerper, Lice antikoerper, Yama antikoerper, mldy antikoerper, xcpp32 antikoerper, casp3 antikoerper, zgc:100890 antikoerper, CASP-3 antikoerper, caspase-3 antikoerper, caspase 3 antikoerper, caspase 3 S homeolog antikoerper, caspase 3, apoptosis-related cysteine peptidase a antikoerper, caspase 3, apoptosis-related cysteine peptidase antikoerper, CASP3 antikoerper, Casp3 antikoerper, casp3.S antikoerper, casp3a antikoerper

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation