SECISBP2 Antikörper (Internal Region)

Produktnummer ABIN185714

Produktdetails anti-SECISBP2 Antikörper

Target: SECISBP2 (SECIS Binding Protein 2 (SECISBP2))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SECISBP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Verwendungszweck: SBP2

Sequenz: KERQERKQRL QEN

Kreuzreaktivität: Rind (Kuh), Hund, Human, Schwein

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: C-KERQERKQRLQEN

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Testis shows granular cytoplasm staining in Leydig cells. Recommended concentration, 3 μg/mL.

Western Blot: Approx 120 kDa band detected in HFF cell lysates. Data kindly provided by Laura Papp and Kum Kum Khanna. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Papp et al, Mol Cell Biol. 2006 J

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunocytochemistry/Immunofluorescence: This antibody has been successfully used in ICC/IF for C33A cells as described in the following paper: Papp et al, Nucleic Acids Res. 2008 Dec,36(22):7192-206, PMID: 19004874.
Immunoprec

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-SECISBP2 Antikörper (ABIN185714)

Raman, Pitts, Seyedali, Hashimoto, Bellinger, Berry: "Selenoprotein W expression and regulation in mouse brain and neurons." in: Brain and behavior, Vol. 3, Issue 5, pp. 562-74, (2014) (PubMed).

Papp, Wang, Kennedy, Boucher, Zhang, Gladyshev, Singh, Khanna: "Functional characterization of alternatively spliced human SECISBP2 transcript variants." in: Nucleic acids research, Vol. 36, Issue 22, pp. 7192-206, (2008) (PubMed).

Details zu SECISBP2

Target: SECISBP2 (SECIS Binding Protein 2 (SECISBP2))

Andere Bezeichnung: SECISBP2 (SECISBP2 Produkte)

Synonyme: SBP2 antikoerper, 2210413N07Rik antikoerper, 2810012K13Rik antikoerper, CG7066 antikoerper, Dmel\\CG7066 antikoerper, DKFZp459F1026 antikoerper, Sbp2 antikoerper, SECIS binding protein 2 antikoerper, SECIS-binding protein 2 antikoerper, Secisbp2 antikoerper, Sbp2 antikoerper, SECISBP2 antikoerper, secisbp2 antikoerper

Hintergrund: SBP2 , RP11-89K14.1, SECISBP2, SECIS binding protein 2 , DKFZp686C09169, OTTHUMP00000064929, OTTHUMP00000064930, OTTHUMP00000064931, OTTHUMP00000064932, selenocysteine insertion sequence binding protein 2

Gen-ID: 79048

NCBI Accession: NP_076982