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Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (AA 429-442), (AA 443-456) Antikörper

Verified NR1H2/3 Reaktivität: Human ELISA, FACS, IF, WB Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN185294
  • Target Alle Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) Produkte
    Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)
    Bindungsspezifität
    AA 429-442, AA 443-456
    Reaktivität
    Human
    Wirt
    Ziege
    Klonalität
    Polyklonal
    Applikation
    ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
    Verwendungszweck
    LXR alpha / LXR beta
    Sequenz
    CRLQDKKLPP LLSEI
    Spezifität
    This antibody is expected to recognise epitopes including aa 429-442 of human LXR alpha protein (NP_005684.2) and aa 443-456 of human LXR beta protein (NP_009052.3).
    Kreuzreaktivität
    Rind (Kuh), Hund, Human, Maus, Ratte
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence CRLQDKKLPPLLSEI, from the internal region of the protein sequence according to NP_005684.2, NP_009052.3.
    Isotyp
    IgG
  • Applikationshinweise

    Western Blot: Approx 55 kDa band observed in Human, Liver and Kidney lysates and in preliminary testing of Human Spleen lysate (calculated MW of 51.1 kDa according to NP_001238863.1). This band was successfully blocked by incubation with the immunizing pe

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Kommentare

    Immunofluorescence: Strong expression of the protein seen in the nuclei of A549 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)
    Andere Bezeichnung
    NR1H3, NR1H2
    Synonyme
    LXR-a antikoerper, LXRA antikoerper, RLD-1 antikoerper, nuclear receptor subfamily 1 group H member 3 antikoerper, NR1H3 antikoerper
    Hintergrund
    NR1H3, LXRA, LXR-a, RLD-1, nuclear receptor subfamily 1, group H, member 3, liver X receptor, alpha, NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor / NR1H2, NER, UNR, LXR-b, N
    Gen-ID
    10062, 7376
    NCBI Accession
    NP_005684, NP_009052, NP_001123573, NP_001238863, NP_001350524
    Pathways
    Nuclear Receptor Transcription Pathway, Cellular Response to Molecule of Bacterial Origin, Hepatitis C
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