V5 Epitope Tag Antikörper

Details zu Produkt Nr. ABIN1842466, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
397
18
17
16
3
3
Wirt
Kaninchen
252
136
34
10
8
7
3
2
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
30
27
21
13
12
12
11
11
11
10
10
10
10
9
9
8
8
7
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
380
276
152
109
88
77
50
45
36
13
9
8
4
4
4
2
2
1
1
1
1
1
Optionen
Hersteller
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Immunogen V5 epitope tag peptide GKPIPNPLLGLDST conjugated to KLH
Sequenz GKPIPNPLLG LDST
Isotyp IgG
Spezifität Rabbit Anti-V5-tag Polyclonal Antibody specifically recognizes V5 tagged fusion proteins.
Produktmerkmale V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
Reinigung Immunoaffinity chromatography
Substanzklasse Tag
Hintergrund The V5 epitope tag is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
Applikationshinweise Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors,
Including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0. 05-0. 2 µg/mL
Western blot: 0. 1-1. 0 µg/mL
Western blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit L00204, C is recommended. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane. Other applications: user-optimized
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
Konzentration 0.5 mg/mL
Buffer lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Avoid repeated freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
Produkt verwendet in: Afrazi, Sodhi, Good, Jia, Siggers, Yazji, Ma, Neal, Prindle, Grant, Branca, Ozolek, Chang, Hackam: "Intracellular heat shock protein-70 negatively regulates TLR4 signaling in the newborn intestinal epithelium." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 9, pp. 4543-57, 2012 (PubMed).

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