Dieser Macrophage Mannose Receptor 1 Antikörper ist konjugiert mit PE
Applikation
Flow Cytometry (FACS)
Spezifität
This antibody recognises the mannose receptor, a 175 kDa type 1 membrane protein that is also known as CD206. Clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands.
Kreuzreaktivität (Details)
Species reactivity (tested):Mouse.
Aufreinigung
Affinity Chromatography on Protein G
Immunogen
Chimaeric CRD4-7-Fc protein. Spleen cells from immunised Fischer rats were fused with cells of the Y3 myeloma cell line.
Flow cytometry: use 10 μL of neat antibody to label 10^6 cells in 100 μL. CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with 1 mL distilled water.
Konzentration
0.1 mg/mL
Buffer
PBS, 0.09 % Sodium Azide, 1 % BSA, 5 % Sucrose
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
Macrophage Mannose Receptor 1 (MRC1)
(Mannose Receptor, C Type 1 (MRC1))
CD206, also known as macrophage mannose receptor type C (MMR or MRC1), is a type I membrane receptor protein. CD206 is a phagocytic and endocytic receptor that can recognize carbohydrate ligands in target molecules. The extracellular portion of the protein includes eight C-type carbohydrate recognition domains (CRD) which are clustered together to achieve higher affinity binding to saccharides. CD206 is found on macrophages and on endothelial cells of the liver and is the only known example of a C-type lectin that contains multiple C-type CRDs. CD206 mediates the endocytosis of glycoproteins by macrophages and binds high-mannose structures on the surface of potentially pathogenic viruses, fungi and bacteria enabling them to be neutralized by phagocytic engulfment. During inflammation, CD206 is crucial for rapid clearance of several mannose-bearing serum glycoproteins but does not regulate the initiation of inflammation. CD206 is primarily expressed in mature tissue macrophages and immature Dendritic cells, as well as hepatic and lymphatic endothelial cells, retinal pigmental epithelium (RPE) and mesangial cells.Synonyms: C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, CLEC13D, CLEC13DL, MRC1L1, Macrophage mannose receptor, Macrophage mannose receptor 1-like protein 1