Dieser CLEC10A Antikörper ist konjugiert mit Biotin
Applikation
Flow Cytometry (FACS)
Spezifität
This antibody recognises the CD301 cell surface protein. In mice, CD301 is predominantly expressed on mature macrophages found associated with a wide range of connective tissues including macrophages in the dermis and the pancreas. Clone ER-MP23 also detects a population of dendritic cells in lymphoid tissue, which are probably recent immigrants from peripheral connective tissue sites. Clone ER-MP23 is reported to block the function of murine CD301. The antibody binds both MGL1 and MGL2 homologues.
Produktmerkmale
Synonyms: CLECSF13, CLECSF14, HML, C-type lectin domain family 10 member A, C-type lectinsuperfamily member 14, Macrophage lectin 2
Aufreinigung
Affinity Chromatography on Protein G
Immunogen
Balb/c macrophage precursor cell hybrids. Cells from immunised rats were fused with cells of the rat Y3-Ag1. 2. 3 myeloma cell line.
CLEC10A
Reaktivität: Maus
FACS
Wirt: Ratte
Monoclonal
LOM-8-7
PE
Applikationshinweise
Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.1 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Haltbarkeit
12 months
Lederle, Hartenstein, Meides, Kunzelmann, Werb, Angel, Mueller: "MMP13 as a stromal mediator in controlling persistent angiogenesis in skin carcinoma." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1175-84, (2010) (PubMed).
Target
CLEC10A
(C-Type Lectin Domain Family 10, Member A (CLEC10A))
CLEC10A antikoerper, CD301 antikoerper, CLECSF13 antikoerper, CLECSF14 antikoerper, HML antikoerper, HML2 antikoerper, MGL antikoerper, CD301a antikoerper, M-ASGP-BP-1 antikoerper, Mgl antikoerper, Mgl1 antikoerper, Mgll antikoerper, C-type lectin domain family 10 member A antikoerper, C-type lectin domain containing 10A antikoerper, C-type lectin domain family 10, member A antikoerper, LOC607405 antikoerper, CLEC10A antikoerper, Clec10a antikoerper
Hintergrund
In mice, CD301 is predominantly expressed on mature macrophages found associated with a wide range of connective tissues including macrophages in the dermis and the pancreas. Clone ER-MP23 also detects a population of dendritic cells in lymphoid tissue, which are probably recent immigrants from peripheral connective tissue sites. Expression of CD301 is induced by alternative (i.e. IL-4/IL-13 mediated) activation of macrophages and dendritic cells, but not all CD301 positive cells are necessarily IL-4/IL-13 stimulated.Synonyms: C-type lectin domain family 10 member A, C-type lectin superfamily member 14, CLECSF13, CLECSF14, HML, Macrophage lectin 2